Sperm characteristics and teratology in rats following vas deferens occlusion with RISUG and its reversal

• Published in: International journal of andrology Vol. 33; no. 1; pp. e198 - e206
• Main Authors: Lohiya, N.K, Suthar, R, Khandelwal, A, Goyal, S, Ansari, A.S, Manivannan, B
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2010; Blackwell Publishing Ltd
• Subjects: Animals; congenital abnormalities; Dimethyl Sulfoxide - pharmacology; DMSO; embryology; Female; Male; male contraception; Maleic Anhydrides - pharmacology; Polyesters - pharmacology; Polystyrenes - pharmacology; Pregnancy; Rats; Rats, Wistar; reversal; RISUG; safety; Spermatozoa - drug effects; Styrene - pharmacology; styrene maleic anhydride; Teratology; Vas Deferens - drug effects; Vasectomy - methods; Wistar albino rat
• ISSN: 0105-6263; 1365-2605
• DOI: 10.1111/j.1365-2605.2009.00992.x

The functional success of the reversal of vas occlusion by styrene maleic anhydride (RISUG), using the solvent vehicle, Dimethyl Sulphoxide (DMSO), has been investigated. Reversal with DMSO was carried out in Wistar albino rats 90 days after bilateral vas occlusion. The body weight, organ weight, sperm characteristics, fertility test and teratology, including skeletal morphology were evaluated in vas occlusion and reversal animals and in F₁ progenies to assess the functional success of the occlusion and reversal. Body weight, organ weight and the cauda epididymal sperm characteristics of vas occlusion and reversal animals and of F₁ progenies were comparable to control. Ejaculated spermatozoa in the vaginal smear showed detached head/tail, acrosomal damage, bent midpiece, bent tail and morphological aberrations in sperm head after vas occlusion, which returned to normal, 90 days after reversal. Monthly fertility test, post-injection showed 0% fertility, which improved gradually and 100% fertility was achieved 90 days after reversal. The fertility/pregnancy/implantation record and skeletal morphology of the offspring were comparable to control. The results suggest functional success and safety of vas occlusion reversal by DMSO.