Maximization of cell growth and lipid production of freshwater microalga Chlorella vulgaris by enrichment technique for biodiesel production

• Published in: Environmental science and pollution research international Vol. 24; no. 10; pp. 9089 - 9101
• Main Authors: Wong, Y.K., Ho, Y.H., Ho, K.C., Leung, H.M., Yung, K.K.L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2017; Springer Nature B.V
• Subjects: Algae; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biodiesel fuels; Biofuels; Biomass; Carotenoids; Cell growth; Chlorella; Chlorella vulgaris; Chlorophyll; Earth and Environmental Science; Eco-Aquaculture; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Fresh Water; Lipids; Lipids - chemistry; Microalgae; Nitrates; Nitrogen; Phosphorus; Productivity; Proteins; Public health; Studies; Sustainable Development and Public Health; Waste Water Technology; Water Management; Water Pollution Control; Culture medium; Lipid production; Algal cultivation; Chlorella vulgaris
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-016-7792-9

Chlorella vulgaris was cultivated under limitation and starvation and under controlled conditions using different concentrations of nitrate (NaNO 3 ) and phosphate (K 2 HPO 4 and KH 2 PO 4 ) chemicals in modified Bold basal medium (BBM). The biomass and lipid production responses to different media were examined in terms of optical density, cell density, dry biomass, and lipid productivity. In the 12-day batch culture period, the highest biomass productivity obtained was 72.083 mg L −1  day −1 under BBM - N control P limited condition. The highest lipid content, lipid concentration, and lipid productivity obtained were 53.202 %, 287.291 mg/L, and 23.449 mg L −1  day −1 under BBM - N Control P Deprivation condition, respectively. Nitrogen had a major effect in the biomass concentration of C . vulgaris , while no significant effect was found for phosphorus. Nitrogen and phosphorus starvation was found to be the strategy affecting the lipid accumulation and affected the lipid composition of C . vulgaris cultures.