Comparative Proteomic Analysis of the Venoms from the Most Dangerous Scorpions in Morocco: Androctonus mauritanicus and Buthus occitanus

Morocco is known to harbor two of the world's most dangerous scorpion species: the black ( ) and the yellow ( ), responsible for 83% and 14% of severe envenomation cases, respectively. Scorpion venom is a mixture of biological molecules of variable structures and activities, most of which are p...

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Published inLife (Basel, Switzerland) Vol. 13; no. 5; p. 1133
Main Authors Hilal, Ines, Khourcha, Soukaina, Safi, Amal, Hmyene, Abdelaziz, Asnawi, Syafiq, Othman, Iekhsan, Stöcklin, Reto, Oukkache, Naoual
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 05.05.2023
MDPI
Subjects
Amines
Androctonus mauritanicus
Biogenic amines
Buthus occitanus
Fractions
High performance liquid chromatography
Ions
Liquid chromatography
Low molecular weights
mass fingerprinting
Mass spectrometry
Mass spectroscopy
Particle size
Peptides
Polyamines
Proteins
Proteomics
Scientific imaging
scorpion
Solvents
Toxins
Venom
Morocco
United States > US
mass fingerprinting
venom
Buthus occitanus
proteomics
mass spectrometry
Androctonus mauritanicus
scorpion
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Summary:Morocco is known to harbor two of the world's most dangerous scorpion species: the black ( ) and the yellow ( ), responsible for 83% and 14% of severe envenomation cases, respectively. Scorpion venom is a mixture of biological molecules of variable structures and activities, most of which are proteins of low molecular weights referred to as toxins. In addition to toxins, scorpion venoms also contain biogenic amines, polyamines, and enzymes. With the aim of investigating the composition of the and venoms, we conducted an analysis of the venoms by mass spectrometry (ESI-MS) after separation by reversed-phase HPLC chromatography. Results from a total of 19 fractions obtained for the venom versus 22 fractions for the venom allowed the identification of approximately 410 and 252 molecular masses, respectively. In both venoms, the most abundant toxins were found to range between 2-5 kDa and 6-8 kDa. This proteomic analysis not only allowed the drawing of an extensive mass fingerprint of the and venoms but also provided a better insight into the nature of their toxins.
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ISSN:2075-1729
2075-1729
DOI:10.3390/life13051133