Unified Theory for Polarization Analysis in Second Harmonic and Sum Frequency Microscopy

A unified theoretical framework for the recovery of second-order nonlinear susceptibility tensors and sample orientations from polarization-dependent second harmonic generation and sum frequency generation microscopy was developed. Jones formalism was extended to nonlinear optics and was used to bri...

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Bibliographic Details
Published inBiophysical journal Vol. 111; no. 7; pp. 1553 - 1568
Main Authors Dow, Ximeng Y., DeWalt, Emma L., Newman, Justin A., Dettmar, Christopher M., Simpson, Garth J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 04.10.2016
Biophysical Society
The Biophysical Society
Subjects
Algorithms
Measurement
Microscopy, Polarization - methods
Models, Theoretical
Optical Phenomena
Optics
Quartz
Quartz - chemistry
Symmetry
Systems Biophysics
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Summary:A unified theoretical framework for the recovery of second-order nonlinear susceptibility tensors and sample orientations from polarization-dependent second harmonic generation and sum frequency generation microscopy was developed. Jones formalism was extended to nonlinear optics and was used to bridge the experimental observables and the local-frame tensor elements. Four commonly used experimental architectures were explicitly explored, including polarization rotation with no postsample optics, polarization-in polarization-out measurement, and polarization modulation with and without postsample optics. Polarization-dependent second harmonic generation measurement was performed on Z-cut quartz and the local-frame tensor elements were calculated. The recovered tensor elements agree with the expected values dictated by symmetry.
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ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2016.04.019