Optical manipulation of single molecules in the living cell
Optical tweezers are the only nano-tools capable of manipulating and performing force-measurements on individual molecules and organelles within the living cell without performing destructive penetration through the cell wall and without the need for inserting a non-endogenous probe. Here, we descri...
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| Published in | Physical chemistry chemical physics : PCCP Vol. 16; no. 25; pp. 12614 - 12624 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.01.2014
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Optical tweezers are the only nano-tools capable of manipulating and performing force-measurements on individual molecules and organelles within the living cell without performing destructive penetration through the cell wall and without the need for inserting a non-endogenous probe. Here, we describe how optical tweezers are used to manipulate individual molecules and perform accurate force and distance measurements within the complex cytoplasm of the living cell. Optical tweezers can grab individual molecules or organelles, if their optical contrast to the medium is large enough, as is the case,
e.g.
, for lipid granules or chromosomes. However, often the molecule of interest is specifically attached to a handle manipulated by the optical trap. The most commonly used handles, their insertion into the cytoplasm, and the relevant micro-rheology of the cell are discussed here and we also review recent and exciting results achieved through optical force manipulation of individual molecules
in vivo
.
Optical tweezers are the only nano-tools capable of manipulating and performing force-measurements on individual molecules and organelles inside the living cell. We present methodologies for
in vivo
calibration and exciting recent results. |
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| Bibliography: | Liselotte Jauffred received her BS degree in mathematics and physics in 2004 and the Cand Scient degree in physics in 2006 from the University of Copenhagen. She graduated as a PhD from the Niels Bohr Institute (NBI) within biophysics in 2010. As a postdoc she has worked at the FOM Institute AMOLF in Amsterdam and is currently a postdoc at NBI. Her research includes interactions between light and quantum dots and she was the first to visualize and quantitate the forces exerted on an optically trapped quantum dot. Kamilla Nørregaard received her BS degree in physics in 2009 and the Cand Scient degree in physics in 2011 from the University of Copenhagen. Currently she is working on her PhD in biophysics under the guidance of Poul M. Bendix and Lene B. Oddershede. Her research interests include plasmonic heating and trapping of nanoparticles, nanoparticle mediated photothermal cancer therapy, and nanoparticle delivered gene therapy. Lene B. Oddershede, PI of the optical tweezers group at the Niels Bohr Institute (NBI), received the Cand Scient degree in mathematics and physics from University of Southern Denmark (SDU) in 1995 and the PhD degree in physics from SDU in 1998. She visited the James Frank Institute, University of Chicago, in 1996-1997. In 1998 she became an assistant professor and constructed the optical tweezers laboratory at NBI, University of Copenhagen. In 2003 she was tenured as an associate professor at the NBI. In 2003 she received the Young Investigator Award from the Danish Optical Society and in 2011 the Silver Medal from the Danish Royal Academy of Sciences. Her research interests include the biophotonical and nanotoxological properties of nanoparticles and their potential use in biological contexts. She is also interested in optical manipulation of biological specimens. Kirstine Berg-Sørensen received the Cand Scient degree in chemistry and physics from University of Aarhus (AU) in 1991 and the PhD degree in physics from AU in 1994. She visited the Laboratoire Kastler-Brossel, École Normale Superieure, Paris, in 1992 and was a postdoc at Rowland Institute for Science, Cambridge MA in 1994-1995. In 1998 she established the optical tweezers group at the NBI with funding from the Danish Research Councils. Since 2005, she has been an associate professor at the Dept of Physics, Technical University of Denmark. In her early research years, she investigated optical trapping and condensation of cold atomic gases whereas more recent research interests involve data analysis in connection with optical manipulation in biological specimens and experiments to do so. In addition, she investigates flow in model systems for plants. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1463-9076 1463-9084 |
| DOI: | 10.1039/c4cp00208c |