Organization and transcription of the gene encoding potato UDP-glucose pyrophosphorylase

• Published in: Gene Vol. 186; no. 2; pp. 293 - 297
• Main Authors: Borovkov, Alex Y, McClean, Phillip E, Secor, Gary A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 28.02.1997
• Subjects: ADN; AGROBACTERIUM; Base Sequence; CARBOHYDRATE METABOLISM; Cloning, Molecular; CODE GENETIQUE; CODIGO GENETICO; DNA; Exons; FOSFOPIROLASA; GENETIC CODE; GENETIC TRANSFORMATION; Introns; METABOLISME DES GLUCIDES; METABOLISMO DE CARBOHIDRATOS; Molecular Sequence Data; PHOSPHORYLASE; Plant; Promoter; Protein Biosynthesis; Recombinant Proteins - biosynthesis; Regulatory Sequences, Nucleic Acid; Restriction Mapping; SOLANUM TUBEROSUM; Solanum tuberosum - enzymology; Solanum tuberosum - genetics; TRANSCRIPCION; TRANSCRIPTION; Transcription, Genetic; TRANSFORMACION GENETICA; Transformation; TRANSFORMATION GENETIQUE; UTP-Glucose-1-Phosphate Uridylyltransferase - biosynthesis; UTP-Glucose-1-Phosphate Uridylyltransferase - genetics; aa, amino acid(s); Glc-1- P, glucose 1-phosphate; Transformation; tsp, transcription start point; cv(s)., cultivar(s); kb, kilobase(s) or 1000 bp; GUS, β-glucuronidase; UTP, uridine 5′-triphosphate; bp, base pair(s); Promoter; Plant; Agrobacterium; nt, nucleotide(s); UGPase, gene (DNA, RNA) encoding UGPase; PCR, polymerase chain reaction; RACE, rapid amplification of cDNA ends; UDP-Glc, uridine 5′-diphosphate glucose; UGPase, UDP-glucose pyrophosphorylase; PP i, pyrophosphate
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(96)00724-X

The organization of the gene encoding potato UDP-glucose pyrophosphorylase, one of the key enzymes of carbohydrate metabolic pathway is presented. The gene cloned from cultivar (cv.) Lemhi consists of a 6.6-kb structural and a 1-kb regulatory region. The structural region contains 20 exons and 19 introns. The coding sequence with exception of three bases is identical with the UGPase cDNA previously cloned from Danshaku-Imo cv. [ Katsube et al. (1990)UDP-Glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. J. Biochem. 108, 321–326]. The largest intron contains a tandem repeat consisting of 50 nt core units. A putative polyadenylation site is situated 79 bp downstream of the translation stop codon. A transcription start point ( tsp) and a putative TATA-box were located 84 bp and 141 bp upstream of the translation start, respectively. The regulatory region contained general enhancer, suppressor, and regions responsible for tissue specificity of UGPase expression.