Intravenous kainic acid induces status epilepticus and late onset seizures in mice

• Published in: Epilepsy research Vol. 178; p. 106816
• Main Authors: Drysdale, Nicolas D., Matthews, Elizabeth, Schuetz, Elizabeth, Pan, Enhui, McNamara, James O.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021
• Subjects: Animals; Disease Models, Animal; Electroencephalography; Epilepsy; Epilepsy, Temporal Lobe - chemically induced; Hippocampus; Intravenous; Kainic acid; Kainic Acid - toxicity; Mice; Mice, Inbred C57BL; Mouse; Seizure; Seizures - chemically induced; Status epilepticus; Status Epilepticus - chemically induced; Seizure; Mouse; Intravenous; Kainic acid; Status epilepticus; Epilepsy
• ISSN: 0920-1211; 1872-6844
• DOI: 10.1016/j.eplepsyres.2021.106816

We set out to establish a novel model of temporal lobe epilepsy (TLE) in a mouse. We sought to induce TLE through the injection of kainic acid (KA) into the tail vein with subsequent development of status epilepticus (SE). Using C57BL/6 mice, we implanted hippocampal EEG recording electrodes before or after injection of KA or phosphate buffered saline (PBS). Video and EEG analysis were conducted to evaluate for SE and development of recurrent seizures, the hallmark of TLE. All mice injected with KA developed SE while those who were injected with PBS did not. Of the animals injected with KA monitored for recurrent seizures following SE, 33% developed spontaneous recurrent seizures while those injected with PBS did not. Injection of KA through the tail vein of a mouse reliably and rapidly induces SE which remits spontaneously and leads to the development of TLE in a subset of mice. •Injection of kainic acid in a mouse via the tail vein rapidly and reliably induces status epilepticus.•Systemic injection of kainic acid via the tail vein in mice shows heterogeneity in SE and seizure development.•Surgical implantations of EEG electrodes prior to kainic acid infusion increases mortality.