The contribution of Ca and Mg to the accumulation of amino acids in maize: from the response of physiological and biochemical processes

• Published in: BMC plant biology Vol. 24; no. 1; pp. 579 - 18
• Main Authors: He, Zhaoquan, Shang, Xue, Wang, Xiukang, Xing, Yingying, Zhang, Tonghui, Yun, Jianying
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.06.2024; BioMed Central; BMC
• Subjects: Abscisic acid; Acetic acid; Amino acid accumulation; Amino acid sequence; Amino acids; Amino Acids - metabolism; Analysis; Antioxidants; Antioxidants - metabolism; Calcium; Calcium - metabolism; Catalase; Cell membranes; Corn; Crop yields; Crops; Defensive enzyme; Driving ability; Enriched uranium; Enzymatic activity; Enzyme activity; Enzymes; Fertilizers; Free radicals; Gene expression; Gibberellins; Gibberellins - metabolism; Hormones; Indoleacetic acid; Indoleacetic Acids - metabolism; Investigations; Kernels; Leucine; Magnesium; Magnesium - metabolism; Maize physiology; Management; Metabolic pathways; Metabolism; Nutrients; Nutritive value; Oxidative stress; Physiological aspects; Physiological effects; Physiology; Plant Growth Regulators - metabolism; Production management; Ripening; Signal transduction; Spraying; Superoxide; Superoxide dismutase; Superoxide Dismutase - metabolism; Synthesis; Waxes; Zea mays - growth & development; Zea mays - metabolism; Zea mays - physiology; Zeatin; Zeatin riboside; China; Maize physiology; Mg; Amino acid accumulation; Ca; Defensive enzyme
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05287-y

The quality of maize kernels is significantly enhanced by amino acids, which are the fundamental building blocks of proteins. Meanwhile, calcium (Ca) and magnesium (Mg), as important nutrients for maize growth, are vital in regulating the metabolic pathways and enzyme activities of amino acid synthesis. Therefore, our study analyzed the response process and changes of amino acid content, endogenous hormone content, and antioxidant enzyme activity in kernels to the coupling addition of sugar alcohol-chelated Ca and Mg fertilizers with spraying on maize. (1) The coupled addition of Ca and Mg fertilizers increased the Ca and Mg content, endogenous hormone components (indole-3-acetic acid, IAA; gibberellin, GA; zeatin riboside, ZR) content, antioxidant enzyme activity, and amino acid content of maize kernels. The content of Ca and Mg in kernels increased with the increasing levels of Ca and Mg fertilizers within a certain range from the filling to the wax ripening stage, and significantly positively correlated with antioxidant enzyme activities. (2) The contents of IAA, GA, and ZR continued to rise, and the activities of superoxide dismutase (SOD) and catalase (CAT) were elevated, which effectively enhanced the ability of cells to resist oxidative damage, promoted cell elongation and division, and facilitated the growth and development of maize. However, the malondialdehyde (MDA) content increased consistently, which would attack the defense system of the cell membrane plasma to some extent. (3) Leucine (LEU) exhibited the highest percentage of essential amino acid components and a gradual decline from the filling to the wax ripening stage, with the most substantial beneficial effect on essential amino acids. (4) CAT and SOD favorably governed essential amino acids, while IAA and MDA negatively regulated them. The dominant physiological driving pathway for the synthesis of essential amino acids was "IAA-CAT-LEU", in which IAA first negatively drove CAT activity, and CAT then advantageously controlled LEU synthesis. These findings provide a potential approach to the physiological and biochemical metabolism of amino acid synthesis, and the nutritional quality enhancement of maize kernel.