Application of micromachine techniques to biotechnological research

• Published in: MATER SCI ENG C BIOMIMETIC SUPRAMOL SYST Vol. 12; no. 1; pp. 67 - 70
• Main Authors: Murakami, Yuji, Idegami, Kotaro, Nagai, Hidenori, Kikuchi, Takayuki, Morita, Yasutaka, Yamamura, Akira, Yokoyama, Kenji, Tamiya, Eiichi
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 01.08.2000; Elsevier Sequoia
• Subjects: Biological and medical sciences; Biosensors; Biotechnological research; Biotechnology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Micromachine techniques; Micromachining; Microsensors; Multianalyte biosensing chip; Various methods and equipments; Micromachine techniques; Multianalyte biosensing chip; Biotechnological research; Biotechnology; Evaluation; Fixation; Support; Technology; Biosensor; Scientific research; Two level method; Biomaterial; Manufacturing
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/S0928-4931(00)00160-0

Novel methods to construct a multianalyte biosensing chip are described. A method is two-step immobilization in which materials are not immobilized directly but indirectly via small support materials. Biomaterials are immobilized on a certain support particle in the first step. The particles are placed or stuck on a chip in the second step. The other method is random fluidic self-assembly of the microsupports for reducing the complication in the second step on the chip. The combination of the methods enables us to immobilize various kinds of biomaterials densely on a chip. The ways to avoid possible problems due to random distribution are discussed. Several examples are also described.