Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice

, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant (MRSA) and multi-drug-resistant (MDR- ) using in vitro and in vivo stu...

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Published inInternational journal of molecular sciences Vol. 24; no. 11; p. 9774
Main Authors Almuhanna, Yasir, Alqasmi, Mohammed Hussein, AlSudais, Hamood, Alrouji, Mohammed, Kuriri, Fahd A, Alissa, Mohammed, Alsuwat, Meshari A, Asad, Mohammed, Joseph, Babu
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 05.06.2023
MDPI
Subjects
Achillea fragrantissima
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Biofilms
Cell lines
Cytotoxicity
Diabetes
Diabetes mellitus
diabetic
Drug resistance
HaCaT cell lines
In vivo methods and tests
Infections
Irritation
LC-MS
Methicillin
Multidrug resistance
Pathogens
Pseudomonas aeruginosa
skin irritation
Staphylococcus aureus
Wound healing
diabetic
LC-MS
HaCaT cell lines
skin irritation
cytotoxicity
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Summary:, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant (MRSA) and multi-drug-resistant (MDR- ) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR- . The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119774