Reduced-Cost Production of Sophorolipids by Starmerella bombicola CGMCC1576 Grown on Cottonseed Molasses and Cottonseed Oil-Based Medium

• Published in: International journal of molecular sciences Vol. 24; no. 6; p. 5759
• Main Authors: Qin, Zehua, Guo, Wei, Liu, Jun, Zhao, Guoqin, Liu, Mingxin, Song, Xin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.03.2023; MDPI
• Subjects: Agricultural production; Biodiesel fuels; Carbon; Carbon sources; Cotton; cottonseed molasses; Cottonseed Oil; Fatty acids; Fermentation; Glucose; Glycolipids - chemistry; Hydrophobicity; Inorganic salts; Molasses; Oil; Oleic Acid; Proteins; Raffinose; Response surface methodology; response surface optimization; Saccharomycetales; sophorolipids; Soybeans; Starmerella bombicola; Substrates; Sucrose; Surfactants; Syrups & sweeteners; Vegetable oils; China; Starmerella bombicola; sophorolipids; cottonseed molasses; cottonseed oil; response surface optimization
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24065759

A large-scale application of sophorolipids (SLs) was blocked by their high production cost. One feasible way to reduce the cost of SL production is to develop cheap feedstocks as the substrates for SL fermentation. In the present work, cottonseed molasses (CM), a waste from raffinose production, was used as the hydrophilic substrate;, and cottonseed oil (CO) was used as a hydrophobic substrate for SL production by CGMCC 1576. The primary optimization of carbon sources, nitrogen source and inorganic salts, produced 57.6 ± 2.3 g/L of total SLs and 24.0 ± 1.2 g/L of lactonic SLs on CM and CO, almost equal to the titer of SLs produced from glucose and oleic. A response surface method was applied to optimize the fermentation medium for growth and SL production of . The production of total SLs reached 58.4 ± 3.4 g/L, and lactonic SLs were elevated to more than 25.0 ± 1.9 g/L. HPLC-MS analysis showed that the compositions of SLs produced by on CM and CO were very similar to those on glucose and oleic acid. These results suggested that cottonseed molasses and cottonseed oil can be used as renewable cheap substrates for the reduced-cost production of SLs.