Production of parathyroid-hormone-related protein by cholesteatoma cells in culture

• Published in: The Lancet (British edition) Vol. 338; no. 8774; pp. 1041 - 1043
• Main Authors: Cheshire, I.M., Blight, A., Ratcliffe, W.A., Proops, D.W., Heath, D.A.
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 26.10.1991; Lancet; Elsevier Limited
• Subjects: Adolescent; Adult; Biological and medical sciences; Bone Diseases - etiology; Bone Diseases - metabolism; Bone Resorption - etiology; Bones; Cholesteatoma - complications; Cholesteatoma - metabolism; Culture Techniques; Disease; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology; Ears & hearing; Female; Humans; Incus; Keratinocytes - metabolism; Male; Medical research; Medical sciences; Middle Aged; Otorhinolaryngology. Stomatology; Parathyroid Hormone-Related Protein; Protein Biosynthesis; Tumors; Proteins; Cell culture; Cholesteatoma; Parathyroid hormone; Pathogenesis; ENT disease; Tumor; Middle ear; Osteolysis
• ISSN: 0140-6736; 1474-547X
• DOI: 10.1016/0140-6736(91)91902-7

Specimens of cholesteatoma were removed at surgery from five patients who had evidence of bone resorption. Parathyroid-hormone-related protein (PTHrP) was detected, by radioimmunoassay, in conditioned media from keratinocyte cultures derived from all five samples. Concentrations of PTHrP in conditioned media from secondary cultures were higher for the cholesteatoma cells than for normal keratinocytes from controls matched for age and sex. Thus, production of PTHrP by cholesteatoma may be a contributory factor in the bone destruction commonly associated with this disorder.