Analysis of somatic mutations in kappa transgenes

• Published in: The Journal of experimental medicine Vol. 172; no. 1; pp. 131 - 137
• Main Authors: Hackett, Jr, J, Rogerson, B J, O'Brien, R L, Storb, U
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 01.07.1990
• Subjects: Animals; Base Sequence; Cell Line; Cloning, Molecular; DNA Mutational Analysis; Gene Rearrangement - genetics; Hybridomas - immunology; Immunoglobulin Variable Region - genetics; Mice; Mice, Transgenic; Molecular Sequence Data; Mutation

We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear...