Analysis of somatic mutations in kappa transgenes

We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear...

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Bibliographic Details
Published inThe Journal of experimental medicine Vol. 172; no. 1; pp. 131 - 137
Main Authors Hackett, Jr, J, Rogerson, B J, O'Brien, R L, Storb, U
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.07.1990
Subjects
Animals
Base Sequence
Cell Line
Cloning, Molecular
DNA Mutational Analysis
Gene Rearrangement - genetics
Hybridomas - immunology
Immunoglobulin Variable Region - genetics
Mice
Mice, Transgenic
Molecular Sequence Data
Mutation
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Summary:We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear matrix association region, kappa enhancer, and C gene were spared. These data indicate that the rearranged kappa gene contains the necessary sequences for targeting of the mutation process, and suggest that the observed localization of mutations to the V region reflects the inherent specificity of this mutation process.
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ISSN:0022-1007
1540-9538
DOI:10.1084/jem.172.1.131