Analysis of somatic mutations in kappa transgenes
We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear...
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| Published in | The Journal of experimental medicine Vol. 172; no. 1; pp. 131 - 137 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
The Rockefeller University Press
01.07.1990
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| Subjects | |
| Online Access | Get full text |
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| Abstract | We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear matrix association region, kappa enhancer, and C gene were spared. These data indicate that the rearranged kappa gene contains the necessary sequences for targeting of the mutation process, and suggest that the observed localization of mutations to the V region reflects the inherent specificity of this mutation process. |
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| AbstractList | We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear matrix association region, kappa enhancer, and C gene were spared. These data indicate that the rearranged kappa gene contains the necessary sequences for targeting of the mutation process, and suggest that the observed localization of mutations to the V region reflects the inherent specificity of this mutation process. |
| Author | Storb, U O'Brien, R L Rogerson, B J Hackett, Jr, J |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2358776$$D View this record in MEDLINE/PubMed |
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| Snippet | We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations... We have examined the nature and localization of somatic mutations in three Kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations... |
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| StartPage | 131 |
| SubjectTerms | Animals Base Sequence Cell Line Cloning, Molecular DNA Mutational Analysis Gene Rearrangement - genetics Hybridomas - immunology Immunoglobulin Variable Region - genetics Mice Mice, Transgenic Molecular Sequence Data Mutation |
| Title | Analysis of somatic mutations in kappa transgenes |
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