Effects of Poly(L-lysine), Poly(acrylic acid) and Poly(ethylene glycol) on the Adhesion, Proliferation and Chondrogenic Differentiation of Human Mesenchymal Stem Cells

• Published in: Journal of biomaterials science. Polymer ed. Vol. 20; no. 5-6; pp. 577 - 589
• Main Authors: Lu, Hongxu, Guo, Likun, Kawazoe, Naoki, Tateishi, Tetsuya, Chen, Guoping
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.01.2009
• Subjects: Acrylic Resins - chemistry; Acrylic Resins - pharmacology; Base Sequence; Cell Adhesion - drug effects; Cell Differentiation - drug effects; CELL FUNCTION; Cell Proliferation - drug effects; Cells, Cultured; Chondrocytes - cytology; Chondrocytes - drug effects; Chondrocytes - physiology; Chondrogenesis - drug effects; CHONDROGENIC DIFFERENTIATION; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Culture Media, Serum-Free; DNA Primers - genetics; Humans; Materials Testing; MESENCHYMAL STEM CELL; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - physiology; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Polylysine - chemistry; Polylysine - pharmacology; Polymerase Chain Reaction; RNA - genetics; RNA - isolation & purification; Solubility; Static Electricity; TISSUE ENGINEERING; Water; WATER-SOLUBLE POLYMER
• ISSN: 0920-5063; 1568-5624
• DOI: 10.1163/156856209X426402

Microenvironments, composed of many kinds of cytokines and growth factors plus extracellular matrices with diverse electrostatic properties, play key roles in controlling cell functions in vivo. In this study, three kinds of water-soluble polymers, positively charged poly(L-lysine) (PLL), negatively charged poly(acrylic acid) (PAAc) and neutral poly(ethylene glycol) (PEG), were compared based on their effects on the adhesion, spread, proliferation and chondrogenic differentiation of human mesenchymal stem cells (MSCs). The MSCs were seeded and cultured in the presence of polymers of different concentrations applied by methods using coating, mixing or covering. The effects of the water-soluble polymers depended on their electrostatic properties and method of application. The methods were in the order of coating, mixing and covering in terms of high to low influence. A low concentration of PLL promoted MSC adhesion, spread, proliferation and chondrogenic differentiation, while a high concentration of PLL was toxic. The PEG-coated surface facilitated cell aggregation and spheroid formation by inhibiting cell adhesion. A high concentration of mixed PEG (10 μg/ml) promoted cell proliferation in serum-free medium. PAAc showed no obvious effects on MSC adhesion, spread, proliferation, or chondrogenic differentiation.