The cacao gene atlas: a transcriptome developmental atlas reveals highly tissue-specific and dynamically-regulated gene networks in Theobroma cacao L

• Published in: BMC plant biology Vol. 24; no. 1; pp. 601 - 23
• Main Authors: Kulesza, Evelyn, Thomas, Patrick, Prewitt, Sarah F, Shalit-Kaneh, Akiva, Wafula, Eric, Knollenberg, Benjamin, Winters, Noah, Esteban, Eddi, Pasha, Asher, Provart, Nicholas, Praul, Craig, Landherr, Lena, dePamphilis, Claude, Maximova, Siela N, Guiltinan, Mark J
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.06.2024; BioMed Central; BMC
• Subjects: Analysis; Anatomy; Arabidopsis thaliana; Botany; Breeding; Breeding methods; Cacao; Cacao - genetics; Cacao - growth & development; Cacao genomics; Chocolate; Cocoa; Crops; Data analysis; Datasets; Development; Developmental stages; Disease resistance; Drought; Farmers; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene mapping; Gene Regulatory Networks; Gene sequencing; Genes; Genes, Plant; Genetic aspects; Genetic research; Genomes; Genomics; Graphical representations; Graphical user interface; Industrial development; Leaves; Life cycle assessment; Methods; Mineral industry; Mining industry; Organ Specificity - genetics; Physiological aspects; Plant biology; Plant breeding; Plant genetics; Plants; Seeds; Statistical analysis; Theobroma cacao; Time measurement; Tissue-specificity; Tissues; Transcriptome; Transcriptome atlas; Transcriptomes; Tree crops; Trees; United States; Transcriptome atlas; Tissue-specificity; Cacao genomics; Gene expression
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05171-9

Theobroma cacao, the cocoa tree, is a tropical crop grown for its highly valuable cocoa solids and fat which are the basis of a 200-billion-dollar annual chocolate industry. However, the long generation time and difficulties associated with breeding a tropical tree crop have limited the progress of breeders to develop high-yielding disease-resistant varieties. Development of marker-assisted breeding methods for cacao requires discovery of genomic regions and specific alleles of genes encoding important traits of interest. To accelerate gene discovery, we developed a gene atlas composed of a large dataset of replicated transcriptomes with the long-term goal of progressing breeding towards developing high-yielding elite varieties of cacao. We describe the creation of the Cacao Transcriptome Atlas, its global characterization and define sets of genes co-regulated in highly organ- and temporally-specific manners. RNAs were extracted and transcriptomes sequenced from 123 different tissues and stages of development representing major organs and developmental stages of the cacao lifecycle. In addition, several experimental treatments and time courses were performed to measure gene expression in tissues responding to biotic and abiotic stressors. Samples were collected in replicates (3-5) to enable statistical analysis of gene expression levels for a total of 390 transcriptomes. To promote wide use of these data, all raw sequencing data, expression read mapping matrices, scripts, and other information used to create the resource are freely available online. We verified our atlas by analyzing the expression of genes with known functions and expression patterns in Arabidopsis (ACT7, LEA19, AGL16, TIP13, LHY, MYB2) and found their expression profiles to be generally similar between both species. We also successfully identified tissue-specific genes at two thresholds in many tissue types represented and a set of genes highly conserved across all tissues. The Cacao Gene Atlas consists of a gene expression browser with graphical user interface and open access to raw sequencing data files as well as the unnormalized and CPM normalized read count data mapped to several cacao genomes. The gene atlas is a publicly available resource to allow rapid mining of cacao gene expression profiles. We hope this resource will be used to help accelerate the discovery of important genes for key cacao traits such as disease resistance and contribute to the breeding of elite varieties to help farmers increase yields.