Catalytic Modification of Porous Two-Dimensional Ni-MOFs on Portable Electrochemical Paper-Based Sensors for Glucose and Hydrogen Peroxide Detection

• Published in: Biosensors (Basel) Vol. 13; no. 5; p. 508
• Main Authors: Yang, Ya, Ji, Wenhui, Yin, Yutao, Wang, Nanxiang, Wu, Wanxia, Zhang, Wei, Pei, Siying, Liu, Tianwei, Tao, Chao, Zheng, Bing, Wu, Qiong, Li, Lin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.04.2023; MDPI
• Subjects: Acids; Biological properties; Biological samples; Biomarkers; Biosensors; Body fluids; Catalysis; Chemical sensors; Diabetes; Disease prevention; electrochemical sensor; Electrochemical Techniques - methods; Electrochemistry; Enzymes; Glucose; Human body; Humans; Hydrogen Peroxide; Inkjet printing; Maternal & child health; Metal-organic frameworks; Microscopy; Ni-MOFs; non-enzymatic catalysis; paper-based sensors; Porosity; Potassium; Screen printing; Sensitivity; Sensors; Spectrum analysis; Voltammetry; Beijing China; China; Ni-MOFs; paper-based sensors; glucose; electrochemical sensor; non-enzymatic catalysis; hydrogen peroxide
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13050508

Rapid and accurate detection of changes in glucose (Glu) and hydrogen peroxide (H O ) concentrations is essential for the predictive diagnosis of diseases. Electrochemical biosensors exhibiting high sensitivity, reliable selectivity, and rapid response provide an advantageous and promising solution. A porous two-dimensional conductive metal-organic framework (cMOF), Ni-HHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene), was prepared by using a one-pot method. Subsequently, it was employed to construct enzyme-free paper-based electrochemical sensors by applying mass-producing screen-printing and inkjet-printing techniques. These sensors effectively determined Glu and H O concentrations, achieving low limits of detection of 1.30 μM and 2.13 μM, and high sensitivities of 5573.21 μA μM cm and 179.85 μA μM cm , respectively. More importantly, the Ni-HHTP-based electrochemical sensors showed an ability to analyze real biological samples by successfully distinguishing human serum from artificial sweat samples. This work provides a new perspective for the use of cMOFs in the field of enzyme-free electrochemical sensing, highlighting their potential for future applications in the design and development of new multifunctional and high-performance flexible electronic sensors.