Fluid Dynamics of the Excretory Flow of Zymogenic and Mucin Contents in Rat Gastric Gland Processed by High-pressure Freezing/Freeze Substitution

The high-pressure freezing/freeze substitution technique followed by Lowi-cryl K4M embedding provided an excellent ultrastructure and retention of antigenicity of rat gastric glands as well as the intraluminal fluid contents. By taking this advantage, we histochemically investigated the excretory fl...

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Published inThe journal of histochemistry and cytochemistry Vol. 50; no. 2; pp. 223 - 234
Main Authors Sawaguchi, Akira, Ishihara, Kazuhiko, Kawano, Jun-ichi, Oinuma, Tsutomu, Hotta, Kyoko, Suganuma, Tatsuo
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.02.2002
SAGE Publications
Subjects
Animals
Enzyme Precursors - secretion
Freeze Substitution
Frozen Sections
Gastric Mucosa - secretion
Immunohistochemistry
Lead
Male
Mucins - secretion
Organometallic Compounds
Potassium Permanganate
Rats
Rats, Wistar
Staining and Labeling
excretory flow
intraluminal channel
gastric gland
zymogenic contents
mucin contents
high-pressure freezing
fluid dynamics
immunogold labeling
permanganate oxidation
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Summary:The high-pressure freezing/freeze substitution technique followed by Lowi-cryl K4M embedding provided an excellent ultrastructure and retention of antigenicity of rat gastric glands as well as the intraluminal fluid contents. By taking this advantage, we histochemically investigated the excretory flow of the zymogenic and mucin contents in rat gastric glandular lumen at the ultrastructural level. The combination of KMnO4-UA/Pb staining for zymogenic contents and Griffonia simplicifolia agglutinin-II (GSA-II) labeling for mucous neck cell (MNC) mucin distinguished the exocytosed zymogenic contents from the MNC mucin in the glandular lumen. Interestingly, at the base and neck regions, the zymogenic contents showed a droplet-like appearance, forming a distinct interface with the MNC mucin. At the pit region, the GSA-II labeling demonstrated restricted paths, designated as MNC mucous channels, which flowed into the surface mucous gel layer. It should be noted that the interface between exocytosed zymogenic contents and MNC mucin disappeared, and that the zymogenic contents merged into the MNC mucous channels. At the top pit region, the surface mucous gel layer showed laminated arrays of three types of gastric mucins. On the basis of these ultrastructural findings, we propose a model of the excretory flow in rat gastric gland.
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ISSN:0022-1554
1551-5044
DOI:10.1177/002215540205000210