Computational characterization of behavioral response of medaka ( Oryzias latipes) treated with diazinon

• Published in: Aquatic toxicology Vol. 71; no. 3; pp. 215 - 228
• Main Authors: Park, Young-Seuk, Chung, Nam-Il, Choi, Kyung-Hee, Cha, Eui Young, Lee, Seung-Kyu, Chon, Tae-Soo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.02.2005; Elsevier Science
• Subjects: analytical methods; animal behavior; Animal, plant and microbial ecology; Animals; Applied ecology; Automated aquatic ecosystem monitoring; Biological and medical sciences; Cluster Analysis; Computational behavior; computer analysis; diazinon; Diazinon - toxicity; Ecotoxicology, biological effects of pollution; Environmental Monitoring - methods; equations; Fast Fourier transform; Fast Fourier transforms; Fourier Analysis; Freshwater; freshwater fish; Fundamental and applied biological sciences. Psychology; General aspects; indicator species; locomotion; Medaka; Motor Activity - drug effects; Oryzias - physiology; Oryzias latipes; Self-organizing map; toxic substances; Water Pollutants, Chemical - toxicity; water pollution; Computational behavior; Medaka; Automated aquatic ecosystem monitoring; Self-organizing map; Fast Fourier transform; Characterization; Freshwater environment; Aquatic environment; Vertebrata; Insecticide; Ecotoxicology; Pisces; Pesticides; Organophosphorus compounds; Behavior; Oryzias latipes; Diazinon
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2004.11.002

The behavior of indicator specimens in response to sub-lethal doses of toxic substances has been used to detect contamination in aquatic ecosystems. Changes in the movement behaviors of medaka ( Oryzias latipes) were analyzed after being treated with diazinon at a concentration of 0.1 mg/l. The movement tracks of medaka were continuously recorded in two-dimension by a digital image processing system both before and after the treatments. Subsequently, two computational methods—two-dimensional fast Fourier transform (2D FFT) and self-organizing map (SOM), were implemented to extract information from the movement data. The differences in the shapes of the movement tracks before and after the treatments were clearly manifested through 2D FFT. The short-distance, irregular turnings in the movement tracks observed after the treatments in the time domain were characteristically transformed to circular or ellipsoidal patterns in the frequency domain. The amplitudes of 2D FFT were efficiently classified by SOM, demonstrating the effects of the different treatments. To evaluate the feasibility of information extraction by 2D FFT, SOM was similarly carried out on the parameters (speed, meander, stop duration, etc.) conventionally used for characterizing the movement tracks. 2D FFT was more efficient in information extraction from the movement data than the parameters. The 2D FFT and SOM were useful as computational methods for automatically detecting response behaviors of indicator specimens exposed to toxic chemicals in aquatic ecosystems.