Genome-Wide Identification and Comparative Transcriptome Methods Reveal FaMDHAR50 Regulating Ascorbic Acid Regeneration and Quality Formation of Strawberry Fruits

• Published in: International journal of molecular sciences Vol. 24; no. 11; p. 9510
• Main Authors: Hou, Guoyan, Yang, Min, He, Caixia, Jiang, Yuyan, Peng, Yuting, She, Musha, Li, Xin, Chen, Qing, Li, Mengyao, Zhang, Yong, Lin, Yuanxiu, Zhang, Yunting, Wang, Yan, He, Wen, Wang, Xiaorong, Tang, Haoru, Luo, Ya
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.05.2023; MDPI
• Subjects: Abiotic stress; Ascorbic acid; ascorbic acid regeneration; Biosynthesis; Catalysis; Chloroplasts; Chromosomes; Citric acid; Cytoplasm; FaMDHAR50; Fragaria; fruit quality; Fruits; Gene expression; Genes; genome-wide analysis; Genomes; Horticulture; Light effects; Localization; Metabolism; Pathogens; Phylogenetics; Plant growth; Proteins; Ripening; Strawberries; strawberry; Stress response; Sucrose; sugar and acid metabolism; Transcriptomes; anthocyanin accumulation; sugar and acid metabolism; genome-wide analysis; FaMDHAR50; strawberry; fruit quality; ascorbic acid regeneration
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24119510

Ascorbic acid (AsA) is a crucial water-soluble antioxidant in strawberry fruit, but limited research is currently available on the identification and functional validation of key genes involved in AsA metabolism in strawberries. This study analyzed the FaMDHAR gene family identification, which includes 168 genes. Most of the products of these genes are predicted to exist in the chloroplast and cytoplasm. The promoter region is rich in cis-acting elements related to plant growth and development, stress and light response. Meanwhile, the key gene that positively regulates AsA regeneration was identified through comparative transcriptome analysis of 'Benihoppe' strawberry (WT) and its natural mutant (MT) with high AsA content (83 mg/100 g FW). The transient overexpression experiment further showed that overexpression of significantly enhanced the AsA content by 38% in strawberry fruit, with the upregulated expression of structural genes involved in AsA biosynthesis ( and ) and recycling and degradation ( , and ) compared with that of the control. Moreover, increased sugar (sucrose, glucose and fructose) contents and decreased firmness and citric acid contents were observed in the overexpressed fruit, which were accompanied by the upregulation of , , and , as well as the downregulation of . Additionally, the content of pelargonidin 3-glucoside markedly decreased, while cyanidin chloride increased significantly. In summary, is a key positive regulatory gene involved in AsA regeneration in strawberry fruit, which also plays an important role in the formation of fruit flavor, apperance and texture during strawberry fruit ripening.