Modulatory effect of omega-3 polyunsaturated fatty acids on osteoblast function and bone metabolism

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 68; no. 6; pp. 387 - 398
• Main Authors: Watkins, Bruce A., Li, Yong, Lippman, Hugh E., Feng, Shulin
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.06.2003
• Subjects: Animals; Bone; Bone and Bones - cytology; Bone and Bones - drug effects; Bone and Bones - enzymology; Bone and Bones - metabolism; Cyclooxygenase 2; Dinoprostone - metabolism; Fatty Acids, Omega-3 - pharmacology; Humans; Isoenzymes - metabolism; Membrane Proteins; Morphogenesis - drug effects; Omega-3 fatty acids; Osteoblast; Osteoblasts - drug effects; Osteoblasts - enzymology; Osteoblasts - physiology; Ovariectomy; PGE 2; Prostaglandin-Endoperoxide Synthases - metabolism; Cbfa1: core binding protein α-1; NO: nitric oxide; BMC: bone mineral content; PGE 2; ALA: α-linolenic acid; PGE 2: prostaglandin E 2; EPA: eicosapentoenoic acid; ALP: alkaline phosphatase; AA: arachidonic acid; DHA: docosahexaenoic acid; GLA: γ-linolenic acid; COX: cyclooxygenase; PUFA: polyunsaturated fatty acids; FSK: forskolin; Osteoblast; Bone; Omega-3 fatty acids; BMD: bone mineral density
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/S0952-3278(03)00063-2

Recent investigations indicate that the type and amount of polyunsaturated fatty acids (PUFA) influence bone formation in animal models and osteoblastic cell functions in culture. In growing rats, supplementing the diet with omega-3 PUFA results in greater bone formation rates and moderates ex vivo prostaglandin E 2 production in bone organ cultures. A protective effect of omega-3 PUFA on minimizing bone mineral loss in ovariectomized rats has also been reported. The actions of omega-3 fatty acids on bone formation appear to be linked to altering osteoblast functions. Herein we describe experiments with MC3T3-E1 osteoblast-like cells that support findings in vivo where omega-3 PUFA modulated COX-2 protein expression, reduced prostaglandin E 2 production, and increased alkaline phosphatase activity. Other studies indicate that the dietary source of PUFA may affect protein expression of Cbfa1 and nodule formation in fetal rat calvarial cells.