Recombinant Ebola virus nucleoprotein and glycoprotein (Gabon 94 strain) provide new tools for the detection of human infections
C Prehaud, E Hellebrand, D Coudrier, VE Volchkov, VA Volchkova, H Feldmann, B Le Guenno and M Bouloy Unite des Arbovirus et virus des Fievres Hemorragiques, Institut Pasteur, Paris, France. After cloning and sequencing the glycoprotein (GP) gene of one of the Gabonese strains of Ebola virus isolated...
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Published in | Journal of general virology Vol. 79; no. 11; pp. 2565 - 2572 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Soc General Microbiol
01.11.1998
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Subjects | |
Online Access | Get full text |
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Summary: | C Prehaud, E Hellebrand, D Coudrier, VE Volchkov, VA Volchkova, H Feldmann, B Le Guenno and M Bouloy
Unite des Arbovirus et virus des Fievres Hemorragiques, Institut Pasteur, Paris, France.
After cloning and sequencing the glycoprotein (GP) gene of one of the
Gabonese strains of Ebola virus isolated during the 1994-1996 outbreak, it
was shown that the circulating virus was of the Zaire subtype. This was
confirmed in this study by cloning and sequencing the nucleoprotein (NP)
gene of this strain. These two structural proteins were also expressed as
recombinant proteins and used in ELISA tests. NP was expressed as a
His-tagged fusion protein in Escherichia coli and was purified on resins
charged with nickel ions. GP was expressed by means of recombinant
baculoviruses in Spodoptera frugiperda cells. Both recombinant proteins
reacted positively in ELISAs for the detection of IgG antibodies in
convalescent human sera from Gabon and Zaire. The difference in the
relative titres of anti-NP and -GP antibodies was variable, depending on
the sera. In addition, the recombinant NP reacted with heterologous sera
from Cote d'Ivoire and was used successfully to detect IgM antibodies by
mu-capture ELISA in sera from Gabonese patients. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-79-11-2565 |