Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation
Powdery mildew is an apple disease caused by the obligate trophic fungus Basic helix-loop-helix (bHLH) transcription factors play important roles in plant development and stress responses, and they have been widely studied in model plants such as However, their role in the stress response of perenni...
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Published in | International journal of molecular sciences Vol. 24; no. 11; p. 9390 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
28.05.2023
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Powdery mildew is an apple disease caused by the obligate trophic fungus
Basic helix-loop-helix (bHLH) transcription factors play important roles in plant development and stress responses, and they have been widely studied in model plants such as
However, their role in the stress response of perennial fruit trees remains unclear. Here, we investigated the role of
in the powdery mildew of apples. The expression of
was significantly induced during the infection of apples with powdery mildew, and the allogenic overexpression of
in
enhanced the resistance to powdery mildew by increasing the accumulation of hydrogen peroxide (H
O
) and activating the salicylic acid (SA) signaling pathway. The transient overexpression of
in apple leaves increased the resistance to powdery mildew. Conversely, when
expression was silenced, the sensitivity of apple leaves to powdery mildew was increased. The physical interaction between MdbHLH093 and MdMYB116 was demonstrated by yeast two-hybrid, bi-molecular fluorescence complementation, and split luciferase experiments. Collectively, these results indicate that MdbHLH093 interacts with MdMYB116 to improve apple resistance to powdery mildew by increasing the accumulation of H
O
and activating the SA signaling pathway, as well as by providing a new candidate gene for resistance molecular breeding. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms24119390 |