Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation

• Published in: International journal of molecular sciences Vol. 24; no. 11; p. 9390
• Main Authors: Ma, Hai, Zou, Fuyan, Li, Dongmei, Wan, Ye, Zhang, Yiping, Zhao, Zhengyang, Wang, Xiping, Gao, Hua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.05.2023; MDPI
• Subjects: Abiotic stress; Accumulation; Acid resistance; Amino acids; apple; Apples; Bacterial infections; Complementation; disease resistance; Fruit trees; Fruits; Fungi; Genes; Hydrogen peroxide; Infections; Leaves; MdbHLH093; Plant diseases; Plant resistance; Powdery mildew; Proteins; Salicylic acid; salicylic acid signal; Signal transduction; Tobacco; Transcription factors; Trees; Yeast; apple; powdery mildew; MdbHLH093; disease resistance; salicylic acid signal; hydrogen peroxide
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24119390

Powdery mildew is an apple disease caused by the obligate trophic fungus Basic helix-loop-helix (bHLH) transcription factors play important roles in plant development and stress responses, and they have been widely studied in model plants such as However, their role in the stress response of perennial fruit trees remains unclear. Here, we investigated the role of in the powdery mildew of apples. The expression of was significantly induced during the infection of apples with powdery mildew, and the allogenic overexpression of in enhanced the resistance to powdery mildew by increasing the accumulation of hydrogen peroxide (H O ) and activating the salicylic acid (SA) signaling pathway. The transient overexpression of in apple leaves increased the resistance to powdery mildew. Conversely, when expression was silenced, the sensitivity of apple leaves to powdery mildew was increased. The physical interaction between MdbHLH093 and MdMYB116 was demonstrated by yeast two-hybrid, bi-molecular fluorescence complementation, and split luciferase experiments. Collectively, these results indicate that MdbHLH093 interacts with MdMYB116 to improve apple resistance to powdery mildew by increasing the accumulation of H O and activating the SA signaling pathway, as well as by providing a new candidate gene for resistance molecular breeding.