The O:34-antigen lipopolysaccharide as an adhesin in Aeromonas hydrophila

• Published in: FEMS microbiology letters Vol. 139; no. 2-3; pp. 97 - 101
• Main Authors: Merino, Susana, Rubires, Xavier, Aguilar, Alicia, Tomás, Juan M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.1996; Blackwell; Oxford University Press
• Subjects: Adhesion; Adhesion to Hep‐2 cells; Aeromonas hydrophila; Aeromonas hydrophila - cytology; Aeromonas hydrophila - genetics; Aeromonas hydrophila serogroup O:34; Antigens; Antigens - physiology; Bacterial Adhesion - physiology; Bacteriology; Biological and medical sciences; Cell Adhesion - genetics; Electrophoresis, Polyacrylamide Gel; Freshwater; Fundamental and applied biological sciences. Psychology; High temperature; Humans; Lipopolysaccharides; Lipopolysaccharides - immunology; Lipopolysaccharides - metabolism; Low temperature; Microbiology; Mutagenesis - physiology; Mutants; O‐antigen LPS; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Transposon mutagenesis; Tumor Cells, Cultured; serogroup O:34; Adhesion to Hep-2 cells; O-antigen LPS; O antigen; Aeromonas hydrophila; Lipopolysaccharide; Bacteria; Host agent relation; Vibrionaceae; Adhesin
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1996.tb08186.x

Abstract We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to adhere to Hep-2 cells. We found a high level of adhesion when the strains were grown at 20 °C but not when they were grown at 37 °C. We previously described that these strains were able to form the O-antigen lipopolysaccharide when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen lipopolysaccharide (rfb mutants), and they showed significantly lower levels of adhesion to Hep-2 cells than the smooth strains. All these results prompted us to conclude that the O-antigen LPS, in these strains, is an important adhesin.