Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules
Summary Background There is upregulation of class II molecules of the major histocompatibility complex (MHC) by keratinocytes in oral squamous cell carcinoma (OSCC) and inflammatory diseases such as lichen planus. The significance of this expression, or whether it is accompanied by upregulation of...
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Published in | British journal of dermatology (1951) Vol. 154; no. 2; pp. 231 - 238 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.02.2006
Blackwell Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Summary
Background There is upregulation of class II molecules of the major histocompatibility complex (MHC) by keratinocytes in oral squamous cell carcinoma (OSCC) and inflammatory diseases such as lichen planus. The significance of this expression, or whether it is accompanied by upregulation of membrane‐bound costimulatory molecules, is unknown.
Objectives To compare the expression of CD40, CD80, CD86, MHC class II and intercellular adhesion molecule‐1 (ICAM‐1), and the ability to induce allogeneic T‐lymphocyte proliferation in vitro, of a CD40– OSCC cell line, its CD40+ transfected derivative and null transfectants.
Methods OSCC cell lines and purified T lymphocytes were cocultured and T cell proliferation recorded. Phenotypes were analysed by flow cytometry.
Results After T lymphocyte proliferation, which all OSCC cell lines were able to induce, there was upregulation of MHC class II and ICAM‐1. However, the CD40+ transfectants were the most immunologically potent and were the only cells to show increased expression of CD86 (as well as further upregulation of CD40 and a statistically insignificant rise in CD80). The effects of blocking antibodies on T‐cell proliferation were only statistically significant with the CD40+ transfectants.
Conclusions While not essential, expression of CD40 by OSCC cells is necessary for optimal induction of allogeneic T lymphocytes, possibly because of concurrent upregulation of other membrane‐bound costimulatory molecules. |
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Bibliography: | ArticleID:BJD7006 ark:/67375/WNG-5WRLCG2R-7 istex:2CFA0187BAA4B76D71BF60474B3EC41E5B0FA553 This study was carried out at the Oral and Maxillofacial Unit, Eastman Dental Institute for Oral Healthcare Sciences, University College London, London WC1 8LD, U.K. Conflicts of interest: None declared. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-0963 1365-2133 |
DOI: | 10.1111/j.1365-2133.2005.07006.x |