Indices of lower airway inflammation in children monosensitized to house dust mite after nasal allergen challenge

• Published in: Allergy Vol. 63; no. 10; pp. 1345 - 1351
• Main Authors: Inal, A, Kendirli, S.G, Yilmaz, M, Altintas, D.U, Karakoc, G.B, Erdogan, S
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Adolescent; Allergies; Animals; Antigens, Dermatophagoides - administration & dosage; Antigens, Dermatophagoides - blood; Antigens, Dermatophagoides - immunology; Asthma; Asthma - diagnosis; Asthma - immunology; Asthma - pathology; Biological and medical sciences; Bronchial Hyperreactivity - diagnosis; Bronchial Hyperreactivity - immunology; Bronchial Hyperreactivity - pathology; Child; children; Children & youth; Dermatology; Dermatophagoides pteronyssinus; Dermatophagoides pteronyssinus - immunology; Dust; Eosinophils - pathology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Hypersensitivity, Immediate - diagnosis; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - pathology; induced sputum; Insects; Leukotrienes - metabolism; Male; Medical sciences; nasal allergen challenge; Nasal Provocation Tests; rhinitis; Rhinitis, Allergic, Seasonal - diagnosis; Rhinitis, Allergic, Seasonal - immunology; Rhinitis, Allergic, Seasonal - pathology; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Skin Tests; Sputum - immunology; Sputum - metabolism; Human; nasal allergen challenge; Lung disease; Allergy; Immunopathology; Nose disease; Rhinitis; Respiratory disease; Dermatology; House dust; Mite; Inflammation; Respiratory system; Asthma; Respiratory tract; Immunology; children; ENT disease; Bronchus disease; Obstructive pulmonary disease; Nasal route; Child; Provocation test; Induced sputum
• ISSN: 0105-4538; 1398-9995; 0108-1675
• DOI: 10.1111/j.1398-9995.2008.01694.x

There are few available data assessing the united airway disease and its systemic aspects in children. With this study, we aimed to investigate the inflammation markers of upper and lower airways before and after nasal allergen challenge in mite sensitive children with different clinical expression of the allergic disease. Four study groups were formed: rhinitis only, without bronchial hyper-responsiveness (R, n = 10), rhinitis with asthma (R + A, n = 22), atopic asymptomatics (AA, n = 8) and nonallergic healthy controls (C, n = 10). Blood eosinophils, nasal and sputum eosinophils, sputum eosinophil cationic protein (ECP) and cys-LTs, and serum ECP levels were measured before and 24 h after nasal allergen challenge. The groups were comparable in terms of age and gender. Cumulative symptom scores recorded during and 1 h after nasal challenge were not significantly different between patients with R, R + A and AA groups. At T₂₄, the children belonging to R, R + A and AA showed significant increases in nasal eosinophils (P < 0.01, P < 0.001, and P = 0.01, respectively), sputum eosinophils (P = 0.01, P < 0.001, and P < 0.05, respectively) and blood eosinophils (P < 0.01, P < 0.001, and P < 0.05, respectively). Similarly, increases in sputum ECP (P < 0.01, P < 0.001, and P = 0.07, respectively) and sputum cys-LT levels (P = 0.07, P < 0.001, and P < 0.05, respectively) were detected in children belonging to these three groups at T₂₄. Sputum eosinophils significantly correlated with blood eosinophils (r = 0.54, P < 0.001) and sputum ECP (r = 0.58, P < 0.001) at T₂₄. This study showed that nasal allergen challenge increased markers of eosinophilic inflammation in both upper and lower airways of children monosensitized to mites, even before the onset of clinical symptoms.