Therapeutic Potential of Magnetic Nanoparticle-Based Human Adipose-Derived Stem Cells in a Mouse Model of Parkinson's Disease

• Published in: International journal of molecular sciences Vol. 22; no. 2; p. 654
• Main Authors: Kim, Ka Young, Chang, Keun-A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.01.2021; MDPI
• Subjects: 6-Hydroxydopamine; Adipose Tissue - cytology; Animal models; Animals; Apomorphine; Behavior; Blood-brain barrier; Brain; Brain - metabolism; Brain - pathology; Cell- and Tissue-Based Therapy - adverse effects; Cell- and Tissue-Based Therapy - methods; Cures; Disease; Disease Models, Animal; Dopamine receptors; Fluorescent Antibody Technique - methods; Hallucinations; human adipose-derived stem cells; Humans; Immunohistochemistry; in vivo imaging; magnetic nanoparticles; Magnetic resonance imaging; Magnetite Nanoparticles; Medical imaging; Mice; Motor ability; Nanoparticles; Neuroimaging; Neurons; Oxidopamine - adverse effects; Parkinson Disease - diagnosis; Parkinson Disease - etiology; Parkinson Disease - therapy; Parkinson's disease; Regeneration; Signs and symptoms; Stem cell transplantation; Stem Cell Transplantation - adverse effects; Stem Cell Transplantation - methods; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Substantia nigra; Transplantation; human adipose-derived stem cells; in vivo imaging; magnetic nanoparticles; Parkinson’s disease
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22020654

Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Several treatments for PD have focused on the management of physical symptoms using dopaminergic agents. However, these treatments induce various adverse effects, including hallucinations and cognitive impairment, owing to non-targeted brain delivery, while alleviating motor symptoms. Furthermore, these therapies are not considered ultimate cures owing to limited brain self-repair and regeneration abilities. In the present study, we aimed to investigate the therapeutic potential of human adipose-derived stem cells (hASCs) using magnetic nanoparticles in a 6-hydroxydopamine (6-OHDA)-induced PD mouse model. We used the Maestro imaging system and magnetic resonance imaging (MRI) for in vivo tracking after transplantation of magnetic nanoparticle-loaded hASCs to the PD mouse model. The Maestro imaging system revealed strong hASCs signals in the brains of PD model mice. In particular, MRI revealed hASCs distribution in the substantia nigra of hASCs-injected PD mice. Behavioral evaluations, including apomorphine-induced rotation and rotarod performance, were significantly recovered in hASCs-injected 6-OHDA induced PD mice when compared with saline-treated counterparts. Herein, we investigated whether hASCs transplantation using magnetic nanoparticles recovered motor functions through targeted brain distribution in a 6-OHDA induced PD mice. These results indicate that magnetic nanoparticle-based hASCs transplantation could be a potential therapeutic strategy in PD.