Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels

Polysaccharides are widely used as building blocks of scaffolds and hydrogels in tissue engineering, which may require their chemical modification to permit crosslinking. The goal of this study was to generate a library of oxidized alginate (oALG) and oxidized hyaluronic acid (oHA) that can be used...

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Published inBiomolecules (Basel, Switzerland) Vol. 10; no. 8; p. 1185
Main Authors Muhammad, Muhammad, Willems, Christian, Rodríguez-Fernández, Julio, Gallego-Ferrer, Gloria, Groth, Thomas
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 14.08.2020
MDPI
Subjects
alginate
Alginic acid
Amino groups
Biocompatibility
Biomedical materials
Caustic soda
Chemical modification
Chitosan
Chloride
Controlled release
Cytotoxicity
Fibroblasts
Hyaluronic acid
Hydrogels
in situ gelling
Molecular weight
Oxidation
Polyethylene glycol
Polysaccharides
Proteins
Reagents
Sodium
Spectrum analysis
Studies
Tissue engineering
United States > US
Germany
alginate
fibroblasts
oxidation
hydrogels
in situ gelling
cytotoxicity
hyaluronic acid
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Summary:Polysaccharides are widely used as building blocks of scaffolds and hydrogels in tissue engineering, which may require their chemical modification to permit crosslinking. The goal of this study was to generate a library of oxidized alginate (oALG) and oxidized hyaluronic acid (oHA) that can be used for in situ gelling hydrogels by covalent reaction between aldehyde groups of the oxidized polysaccharides (oPS) and amino groups of carboxymethyl chitosan (CMC) through imine bond formation. Here, we studied the effect of sodium periodate concentration and reaction time on aldehyde content, molecular weight of derivatives and cytotoxicity of oPS towards 3T3-L1 fibroblasts. It was found that the molecular weights of all oPs decreased with oxidation and that the degree of oxidation was generally higher in oHA than in oALG. Studies showed that only oPs with an oxidation degree above 25% were cytotoxic. Initial studies were also done on the crosslinking of oPs with CMC showing with rheometry that rather soft gels were formed from higher oxidized oPs possessing a moderate cytotoxicity. The results of this study indicate the potential of oALG and oHA for use as in situ gelling hydrogels or inks in bioprinting for application in tissue engineering and controlled release.
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ISSN:2218-273X
2218-273X
DOI:10.3390/biom10081185