miR-27a-5p Attenuates Hypoxia-induced Rat Cardiomyocyte Injury by Inhibiting Atg7

• Published in: International journal of molecular sciences Vol. 20; no. 10; p. 2418
• Main Authors: Zhang, Jinwei, Qiu, Wanling, Ma, Jideng, Wang, Yujie, Hu, Zihui, Long, Keren, Wang, Xun, Jin, Long, Tang, Qianzi, Tang, Guoqing, Zhu, Li, Li, Xuewei, Shuai, Surong, Li, Mingzhou
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.05.2019; MDPI
• Subjects: acute myocardial infarction; AKT protein; Animals; Apoptosis; Autophagy; Autophagy-Related Protein 7 - antagonists & inhibitors; Calcineurin; Cardiomyocytes; Cardiovascular disease; Cell death; Cell differentiation; Cell Line; Congestive heart failure; Coronary artery; Coronary artery disease; Coronary vessels; Disease Models, Animal; Down-Regulation; FOXO3 protein; Gene Expression Regulation; Heart failure; Heart Injuries; Hypertrophy; Hypoxia; Hypoxia - metabolism; Injury prevention; Male; MicroRNAs; MicroRNAs - metabolism; MicroRNAs - pharmacology; miR-27a-5p; miRNA; Myocardial Infarction - metabolism; Myocardium; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; NF-AT protein; Nutrients; Occlusion; Phagocytosis; Poly(ADP-ribose) polymerase; Protein expression; Proteins; Rats; Rats, Sprague-Dawley; Roles; SIRT1 protein; Starvation; Survival; TOR protein; hypoxia; apoptosis; autophagy; miR-27a-5p; acute myocardial infarction
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20102418

Acute myocardial infarction (AMI) is an ischemic heart disease with high mortality worldwide. AMI triggers a hypoxic microenvironment and induces extensive myocardial injury, including autophagy and apoptosis. MiRNAs, which are a class of posttranscriptional regulators, have been shown to be involved in the development of ischemic heart diseases. We have previously reported that hypoxia significantly alters the miRNA transcriptome in rat cardiomyoblast cells (H9c2), including miR-27a-5p. In the present study, we further investigated the potential function of miR-27a-5p in the cardiomyocyte response to hypoxia, and showed that miR-27a-5p expression was downregulated in the H9c2 cells at different hypoxia-exposed timepoints and the myocardium of a rat AMI model. Follow-up experiments revealed that miR-27a-5p attenuated hypoxia-induced cardiomyocyte injury by regulating autophagy and apoptosis via , which partly elucidated the anti-hypoxic injury effects of miR-27a-5p. Taken together, this study shows that miR-27a-5p has a cardioprotective effect on hypoxia-induced H9c2 cell injury, suggesting it may be a novel target for the treatment of hypoxia-related heart diseases.