Biodegradable 3D Printed Scaffolds of Modified Poly (Trimethylene Carbonate) Composite Materials with Poly (L-Lactic Acid) and Hydroxyapatite for Bone Regeneration

• Published in: Nanomaterials (Basel, Switzerland) Vol. 11; no. 12; p. 3215
• Main Authors: Kang, Honglei, Jiang, Xudong, Liu, Zhiwei, Liu, Fan, Yan, Guoping, Li, Feng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.11.2021; MDPI
• Subjects: 3-D printers; Acids; Biocompatibility; Biodegradability; Biodegradation; Biomaterials; Biomedical materials; Bone biomaterials; Bone growth; Calorimetry; Cell differentiation; Cell proliferation; Composite materials; Contact angle; Cytotoxicity; Differential scanning calorimetry; Fourier transforms; Hemodialysis; Hydroxyapatite; In vivo methods and tests; Infrared spectroscopy; Liquid chromatography; Molecular weight; Multiplication; Nanoparticles; Photomicrographs; poly (L-lactic acid); poly (trimethylene carbonate); Polylactic acid; Polymers; Regeneration; Regeneration (physiology); Scaffolds; Thermogravimetry; Three dimensional printing; Tissue engineering; Toxicity; Transplantation; Transplants & implants; United States > US; China; biodegradability; hydroxyapatite; cell proliferation; poly (trimethylene carbonate); poly (L-lactic acid)
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano11123215

Biodegradable scaffolds based on biomedical polymeric materials have attracted wide interest in bone transplantation for clinical treatment for bone defects without a second operation. The composite materials of poly(trimethylene carbonate), poly(L-lactic acid), and hydroxyapatite (PTMC/PLA/HA and PTMC/HA) were prepared by the modification and blending of PTMC with PLA and HA, respectively. The PTMC/PLA/HA and PTMC/HA scaffolds were further prepared by additive manufacturing using the biological 3D printing method using the PTMC/PLA/HA and PTMC/HA composite materials, respectively. These scaffolds were also characterized by Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), automatic contact-angle, scanning electronic micrographs (SEM), diffraction of X-rays (XRD), differential scanning calorimetry (DSC), and thermogravimetry (TG). Subsequently, their properties, such as mechanical, biodegradation, cell cytotoxicity, cell compatibility in vitro, and proliferation/differentiation assay in vivo, were also investigated. Experiment results indicated that PTMC/PLA/HA and PTMC/HA scaffolds possessed low toxicity, good biodegradability, and good biocompatibility and then enhanced the cell multiplication ability of osteoblast cells (MC3T3-E1). Moreover, PTMC/PLA/HA and PTMC/HA scaffolds enhanced the adhesion and proliferation of MC3T3-E1 cells and enabled the bone cell proliferation and induction of bone tissue formation. Therefore, these composite materials can be used as potential biomaterials for bone repatriation and tissue engineering.