Identification of O-glycosylated proteins that are aberrantly excreted in the urine of patients with early stage ovarian cancer

Cancer is known to induce or alter the O-glycosylation of selective proteins that may eventually be excreted in the patients' urine. The present study was performed to identify O-glycosylated proteins that are aberrantly excreted in the urine of patients with early stage ovarian cancer (OCa). T...

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Published inInternational journal of molecular sciences Vol. 14; no. 4; pp. 7923 - 7931
Main Authors Mu, Alan Kang-Wai, Lim, Boon-Kiong, Hashim, Onn Haji, Shuib, Adawiyah Suriza
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 11.04.2013
Molecular Diversity Preservation International (MDPI)
Subjects
Aged
Aged, 80 and over
biomarker
Biomarkers, Tumor - urine
champedak galactose binding lectin
Clusterin - urine
Female
Glycoproteins - urine
Glycosylation
Humans
Kininogens - urine
Middle Aged
Neoplasm Proteins - urine
Neoplasm Staging
O-glycan
Ovarian cancer
Ovarian Neoplasms - pathology
Ovarian Neoplasms - urine
proteomics
two-dimensional gel electrophoresis
urine
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Summary:Cancer is known to induce or alter the O-glycosylation of selective proteins that may eventually be excreted in the patients' urine. The present study was performed to identify O-glycosylated proteins that are aberrantly excreted in the urine of patients with early stage ovarian cancer (OCa). These urinary glycoproteins are potential biomarkers for early detection of OCa. In this study, urinary proteins of patients with early stage OCa and age-matched OCa negative women were subjected to two-dimensional gel electrophoresis and detection using a lectin that binds to the O-glycosylated proteins. Our analysis demonstrated significant enhanced expression of clusterin and leucine-rich alpha-2-glycoprotein, but lower levels of kininogen in the urine of the OCa patients compared to the controls. The different altered levels of these urinary glycoproteins were further confirmed using competitive ELISA. Our data are suggestive of the potential use of the aberrantly excreted urinary O-glycosylated proteins as biomarkers for the early detection of OCa, although this requires further validation in a large clinically representative population.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms14047923