Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation

• Published in: International journal of molecular sciences Vol. 19; no. 5; p. 1424
• Main Authors: Adnan, Miaad, Li, Kai, Wang, Jianhua, Xu, Li, Yan, Yunjun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.05.2018; MDPI
• Subjects: adsorption method; Biodiesel fuels; biodiesel production; Biofuels; Burkholderia cepacia; Burkholderia cepacia - enzymology; Burkholderia cepacia lipase (BCL); Catalysis; Cetrimonium Compounds - chemistry; Diesel; Enzymes, Immobilized - chemistry; Esterification; hexahedral ZIF-8; hierarchical ZIF-8; Histidine; Histidine - chemistry; Immobilization; Lipase; Lipase - chemistry; Metal-organic frameworks; surfactant; Transesterification; Zeolites - chemistry; biodiesel production; hierarchical ZIF-8; Burkholderia cepacia lipase (BCL); hexahedral ZIF-8; surfactant; adsorption method
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19051424

A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.