Interaction of a Novel Zn2Cys6 Transcription Factor DcGliZ with Promoters in the Gliotoxin Biosynthetic Gene Cluster of the Deep-Sea-Derived Fungus Dichotomomyces cejpii

• Published in: Biomolecules (Basel, Switzerland) Vol. 10; no. 1; p. 56
• Main Authors: Huang, Zi-Lei, Ye, Wei, Zhu, Mu-Zi, Kong, Ya-Li, Li, Sai-Ni, Liu, Shan, Zhang, Wei-Min
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.12.2019; MDPI
• Subjects: Binding sites; Biosynthesis; Carbon; Cytochrome; dcgliz regulator; Deep sea; dichotomomyces cejpii; DNA methylation; Electrophoretic mobility; electrophoretic mobility shift assay; Enzymes; Fungi; Fungi - genetics; Fungi - metabolism; Genes; Genomes; GLI gene; Gliotoxin; Gliotoxin - biosynthesis; Gliotoxin - chemistry; Inclusion bodies; Multigene Family - genetics; Phylogenetics; Promoter Regions, Genetic - genetics; Promoters; Proteins; Regulation; Sulfur; Surface plasmon resonance; Transcription factors; Transcription Factors - metabolism; United States > US; Dichotomomyces cejpii; electrophoretic mobility shift assay; DcGliZ regulator; gliotoxin biosynthesis; surface plasmon resonance
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom10010056

Gliotoxin is an important epipolythiodioxopiperazine, which was biosynthesized by the gene cluster in . However, the regulatory mechanism of gliotoxin biosynthesis remains unclear. In this study, a novel Zn2Cys6 transcription factor DcGliZ that is responsible for the regulation of gliotoxin biosynthesis from the deep-sea-derived fungus was identified. DcGliZ was expressed in and effectively purified from inclusion bodies by refolding. Using electrophoretic mobility shift assay, we demonstrated that purified DcGliZ can bind to g , , and promoter regions in the cluster. Furthermore, the binding kinetics and affinity of DcGliZ protein with different promoters were measured by surface plasmon resonance assays, and the results demonstrated the significant interaction of DcGliZ with the and promoters. These new findings would lay the foundation for the elucidation of future gliotoxin biosynthetic regulation mechanisms in .