Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase

• Published in: International journal of molecular sciences Vol. 21; no. 10; p. 3588
• Main Authors: Jia, Yang, Eltoukhy, Adel, Wang, Junhuan, Li, Xianjun, Hlaing, Thet Su, Aung, Mar Mar, Nwe, May Thet, Lamraoui, Imane, Yan, Yanchun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.05.2020; MDPI
• Subjects: Bacteria; Benzhydryl Compounds - metabolism; Biodegradation; Biodegradation, Environmental; Bisphenol A; Carbon; Cytochrome; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Cytochrome P450 monooxygenase; Cytochromes P450; Efficiency; Enzymes; Genes; Hydrogen-Ion Concentration; Metabolism; Metabolites; Metabolome; Microorganisms; Phenols - metabolism; Phylogenetics; Phylogeny; Pollutants; Retention; Soil microorganisms; Soil Pollutants; Soil pollution; Soil remediation; Sphingobium; Sphingobium sp; Sphingomonadaceae - enzymology; Sphingomonadaceae - genetics; Temperature; Sphingobium sp; biodegradation; bisphenol A; cytochrome P450; soil remediation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21103588

Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by sp. YC-JY1. BL21 (DE3) cells harboring pET28a- achieved the ability to degrade BPA. The gene knockout strain YC-JY1Δ was unable to degrade BPA indicating that P450 was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.