Tear-Derived Exosome Proteins Are Increased in Patients with Thyroid Eye Disease

• Published in: International journal of molecular sciences Vol. 22; no. 3; p. 1115
• Main Authors: Han, Jeong-Sun, Kim, Sung Eun, Jin, Jun-Qing, Park, Na Ri, Lee, Ji-Young, Kim, Hong Lim, Lee, Seong-Beom, Yang, Suk-Woo, Lim, Dong-Jun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.01.2021; MDPI
• Subjects: Biomarkers; C-reactive protein; Chitinase; Cytokines; Deoxyribonucleic acid; DNA; eear fluids; ehyroid-associated ophthalmopathy; Exosomes; extracellular vesicle; Eye diseases; eytokines; Fibroblasts; Fluids; Gelatinase B; Graves’ ophthalmopathy; Inflammation; Interleukin 8; Lipids; Matrix metalloproteinase; Matrix metalloproteinases; Metalloproteinase; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Monocytes; Nanoparticles; Pathogenesis; Proteins; Proteomes; Thyroid; Thyroid gland; Tumor necrosis factor-TNF; Vascular cell adhesion molecule 1; Vitamin D; eytokines; Graves’ ophthalmopathy; extracellular vesicle; exosomes; eear fluids; ehyroid-associated ophthalmopathy
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22031115

Exosomes contain proteins, lipids, RNA, and DNA that mediate intercellular signaling. Exosomes can contribute to the pathological processes of various diseases, although their roles in ocular diseases are unclear. We aimed to isolate exosomes from tear fluids (TF) of patients with Thyroid eye disease (TED) and analyze the exosomal proteins. TFs were collected from eight patients with TED and eight control subjects. The number of TF exosomes were measured using nanoparticle-tracking analysis. The expression of specific proteins in the purified exosome pellets were analyzed using a Proteome Profiler Array Kit. Cultured normal orbital fibroblasts were incubated with TF exosomes from patients with TED and control subjects, and changes in inflammatory cytokine levels were compared. TF exosomes from TED patients showed more exosomes than the control subjects. The expression levels of exosomal proteins vitamin D-binding (VDB) protein, C-reactive protein (CRP), chitinase 3-like 1 (CHI3L1), matrix metalloproteinase-9 (MMP-9), and vascular adhesion molecule-1 (VCAM-1) were significantly increased in patients with TED, compared to those of controls. Orbital fibroblasts exposed to TF exosomes from patients with TED showed significantly higher levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1) production than those treated with control TF exosomes. Specific proteins showed higher expression in exosomes from TED patients, implying that they may play keys roles in TED pathogenesis.