Anti-streptococcal, tubulin, and dopamine receptor 2 antibodies in children with PANDAS and Tourette syndrome: Single-point and longitudinal assessments

• Published in: Journal of neuroimmunology Vol. 264; no. 1-2; pp. 106 - 113
• Main Authors: Morris-Berry, C.M., Pollard, M., Gao, S., Thompson, C., Singer, H.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2013
• Subjects: Adolescent; Allergy and Immunology; Anti-neuronal antibodies; Antibodies - blood; Bacterial Proteins - immunology; Child; D2 receptor; Deoxyribonucleases - immunology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Longitudinal Studies; Male; Neurology; PANDAS; Polyendocrinopathies, Autoimmune - blood; Polyendocrinopathies, Autoimmune - complications; Polyendocrinopathies, Autoimmune - immunology; Receptors, Dopamine D2 - immunology; Single-point and longitudinal; Streptolysins - immunology; Tourette syndrome; Tourette Syndrome - blood; Tourette Syndrome - complications; Tubulin; Tubulin - immunology; D2 receptor; Tubulin; Tourette syndrome; PANDAS; Single-point and longitudinal; Anti-neuronal antibodies
• ISSN: 0165-5728; 1872-8421; 1872-8421
• DOI: 10.1016/j.jneuroim.2013.09.010

Single-point-in-time ELISA optical densities for three putative antibodies identified in Sydenham's chorea, the streptococcal group A carbohydrate antigen, N-acetyl-beta-d-glucosamine, tubulin, and the dopamine 2 receptor, showed no differences in children with PANDAS (n=44) or Tourette syndrome (n=40) as compared to controls (n=24). Anti-tubulin and D2 receptor antibodies assessed in serial samples from 12 PANDAS subjects obtained prior to a documented exacerbation, during the exacerbation (with or without a temporally associated streptococcal infection), and following the exacerbation, showed no evidence of antibody levels correlating with a clinical exacerbation. These data do not support hypotheses suggesting an autoimmune hypothesis in either TS or PANDAS. •Antibodies evaluated in TS and PANDAS children included GlcNAc, tubulin, and the D2R.•Single-point ELISA OD showed no differences in PANDAS or TS compared to controls.•Serial PANDAS antibody levels did not correlate with a clinical exacerbation.•Neither ASO nor anti-DNase B levels correlated with antibody markers.