Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. by high-speed counter-current chromatography

• Published in: Journal of Chromatography A Vol. 1052; no. 1; pp. 217 - 221
• Main Authors: Liu, Renmin, Li, Aifeng, Sun, Ailing
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.10.2004; Elsevier
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Anthraquinones - isolation & purification; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Cinnamates - chemistry; Cinnamic acid; Countercurrent Distribution - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General pharmacology; High-speed counter-current chromatography; Hydrogen-Ion Concentration; Hydroxyanthraquinones; Magnetic Resonance Spectroscopy; Medical sciences; Other chromatographic methods; pH-gradient elution; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Reference Standards; Rheum - chemistry; Rheum officinale Baill; Asia; China; Rheum officinale Baill; Hydroxyanthraquinones; Cinnamic acid; pH-gradient elution; High-speed counter-current chromatography; Countercurrent chromatography; Elution; pH gradient; Purification; Pharmacognosy; Polygonaceae; Preparative chromatography; Medicinal plant; Dicotyledones; Cinnamic acid derivatives; Angiospermae; Plant origin; Isolation; Spermatophyta
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.08.101

A high-speed counter-current chromatography (HSCCC) method for preparative separation and purification of five hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. was developed by using pH-gradient elution. The purities of rhein, emodin, aloe-emodin, chrysophanol, physcion and cinnamic acid were all over 98%, as determined by high performance liquid chromatography (HPLC). The structures of them were identified by 1H NMR.