Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescence In Situ Hybridization

The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluoresence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antig...

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Published inThe journal of histochemistry and cytochemistry Vol. 45; no. 3; pp. 375 - 382
Main Authors van Gijlswijk, Rob P. M, Zijlmans, Henry J. M. A. A, Wiegant, Joop, Bobrow, Mark N, Erickson, Thomas J, Adler, Karl E, Tanke, Hans J, Raap, Anton K
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.03.1997
SAGE Publications
Subjects
Animals
Antigens - analysis
Fluorescent Dyes - chemistry
Horseradish Peroxidase
Humans
Immunoenzyme Techniques
Immunohistochemistry - methods
In Situ Hybridization, Fluorescence - methods
Rats
Sensitivity and Specificity
Tyramine - chemistry
Vimentin - analysis
TSA
fluorescence
peroxidase
antibodies
immunohistochemistry
tyramide
in situ hybridization
oligonucleotide
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Summary:The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluoresence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions, all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISH methods. (J Histochem Cytochem 45:375–382, 1997)
Bibliography:ObjectType-Article-1
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ISSN:0022-1554
1551-5044
DOI:10.1177/002215549704500305