High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL

• Published in: Genes Vol. 9; no. 1; p. 19
• Main Authors: Dhariwal, Raman, Fedak, George, Dion, Yves, Pozniak, Curtis, Laroche, André, Eudes, François, Randhawa, Harpinder Singh
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.01.2018; MDPI
• Subjects: Deoxynivalenol; Epistasis; Ergot; Fusarium; Fusarium head blight; Gene mapping; Genotype & phenotype; Genotyping; Lodging; Quantitative trait loci; Single-nucleotide polymorphism; disease resistance; epistasis; ergot; plant height; doubled haploid; grain yield; grain protein content; Fusarium head blight; test weight
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes9010019

Triticale (x Wittmack) is an important feed crop which suffers severe yield, grade and end-use quality losses due to head blight (FHB). Development of resistant triticale cultivars is hindered by lack of effective genetic resistance sources. To dissect FHB resistance, a doubled haploid spring triticale population produced from the cross TMP16315/AC Ultima using a microspore culture method, was phenotyped for FHB incidence, severity, visual rating index (VRI), deoxynivalenol (DON) and some associated traits (ergot, grain protein content, test weight, yield, plant height and lodging) followed by single nucleotide polymorphism (SNP) genotyping. A high-density map consisting of 5274 SNPs, mapped on all 21 chromosomes with a map density of 0.48 cM/SNP, was constructed. Together, 17 major quantitative trait loci were identified for FHB on chromosomes 1A, 2B, 3A, 4A, 4R, 5A, 5R and 6B; two of incidence loci (on 2B and 5R) also co-located with loci for severity and VRI, and two other loci of VRI (on 1A and 4R) with DON accumulation. Major and minor loci were also identified for all other traits in addition to many epistasis loci. This study provides new insight into the genetic basis of FHB resistance and their association with other traits in triticale.