RNA-Seq Analysis Identifies Transcription Factors Involved in Anthocyanin Biosynthesis of 'Red Zaosu' Pear Peel and Functional Study of PpPIF8

• Published in: International journal of molecular sciences Vol. 23; no. 9; p. 4798
• Main Authors: Ma, Zhenyu, Wei, Chuangqi, Cheng, Yudou, Shang, Zhonglin, Guo, Xiulin, Guan, Junfeng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.04.2022; MDPI
• Subjects: anthocyanin; Anthocyanins; Anthocyanins - metabolism; Biosynthesis; Clustering; Coloration; Correlation analysis; Flavonoids; Flowers & plants; Fruit - metabolism; Fruits; Gene expression; Gene Expression Regulation, Plant; Genomes; Plant Proteins - genetics; Plant Proteins - metabolism; PpPIF8; Pyrus; Pyrus - metabolism; Pyrus communis; RNA-Seq; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptomes; WGCNA; PpPIF8; anthocyanin; RNA-Seq; transcription factors; WGCNA; Pyrus
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23094798

Red-skinned pears are favored by people for their attractive appearance and abundance of anthocyanins. However, the molecular basis of anthocyanin biosynthesis in red pears remains elusive. Here, a comprehensive transcriptome analysis was conducted to explore the potential regulatory mechanism of anthocyanin biosynthesis in 'Red Zaosu' pear ( × ). Gene co-expression analysis and transcription factor mining identified 263 transcription factors, which accounted for 6.59% of the total number of transcription factors in the pear genome in two gene modules that are highly correlated with anthocyanin biosynthesis. Clustering, gene network modeling with STRING-DB, and local motif enrichment analysis (CentriMo) analysis suggested that PpPIF8 may play a role in anthocyanin biosynthesis. Furthermore, eight PIFs were identified in the pear genome, of which only was rapidly induced by light. Functional studies showed that PpPIF8 localizes in the nucleus and is preferentially expressed in the tissue of higher levels of anthocyanin. The overexpression of PpPIF8 in pear peel and pear calli promotes anthocyanin biosynthesis and upregulates the expression of anthocyanin biosynthesis genes. Yeast-one hybrid and transgenic analyses indicated that PpPIF8 binds to the promoter to induce expression. The positive effect of PpPIF8 on anthocyanin biosynthesis is different from previously identified negative regulators of PyPIF5 and MdPIF7 in pear and apple. Taken together, our data not only provide a comprehensive view of transcription events during the coloration of pear peel, but also resolved the regulatory role of PpPIF8 in the anthocyanin biosynthesis pathway.