Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

• Published in: International journal of molecular sciences Vol. 15; no. 3; pp. 3624 - 3639
• Main Authors: Lin, Rong-Jyh, Wu, Mei-Hsuan, Ma, Yi-Hsuan, Chung, Li-Yu, Chen, Chung-Yi, Yen, Chuan-Min
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.02.2014; Molecular Diversity Preservation International (MDPI)
• Subjects: Alkaloids - isolation & purification; Alkaloids - pharmacology; Animals; Anisakis - drug effects; Anisakis - physiology; Anisakis simplex; anthelmintic activity; Anthelmintics - isolation & purification; Anthelmintics - pharmacology; aporphine; Aporphines - isolation & purification; Aporphines - pharmacology; Aquatic plants; Biological activity; Drugs, Chinese Herbal - pharmacology; Herbal medicine; Hymenolepis nana; Hymenolepis nana - drug effects; Hymenolepis nana - physiology; Movement - drug effects; Nelumbo - chemistry; Nelumbo nucifera Gaertn; Nematodes; peroxyl radical; Plant Leaves - chemistry; Time Factors
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms15033624

Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.