Temperature Effects on Force and Actin⁻Myosin Interaction in Muscle: A Look Back on Some Experimental Findings

• Published in: International journal of molecular sciences Vol. 19; no. 5; p. 1538
• Main Author: Ranatunga, K W
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.05.2018; MDPI
• Subjects: Actin; Actins - metabolism; Adenosine; Adenosine diphosphate; Adenosine triphosphatase; Adenosine Triphosphate - metabolism; Animals; Heart diseases; Humans; Isometric Contraction; Muscle contraction; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Muscles; Myosin; Myosins - metabolism; Review; Temperature; Temperature dependence; Temperature effects; crossbridge force; crossbridge cycle; endothermic force; muscle force; muscle shortening; actin–myosin; temperature-sensitivity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19051538

Observations made in temperature studies on mammalian muscle during force development, shortening, and lengthening, are re-examined. The isometric force in active muscle goes up substantially on warming from less than 10 °C to temperatures closer to physiological (>30 °C), and the sigmoidal temperature dependence of this force has a half-maximum at ~10 °C. During steady shortening, when force is decreased to a steady level, the sigmoidal curve is more pronounced and shifted to higher temperatures, whereas, in lengthening muscle, the curve is shifted to lower temperatures, and there is a less marked increase with temperature. Even with a small rapid temperature-jump (T-jump), force in active muscle rises in a definitive way. The rate of tension rise is slower with adenosine diphosphate (ADP) and faster with increased phosphate. Analysis showed that a T-jump enhances an early, pre-phosphate release step in the acto-myosin (crossbridge) ATPase cycle, thus inducing a force-rise. The sigmoidal dependence of steady force on temperature is due to this endothermic nature of crossbridge force generation. During shortening, the force-generating step and the ATPase cycle are accelerated, whereas during lengthening, they are inhibited. The endothermic force generation is seen in different muscle types (fast, slow, and cardiac). The underlying mechanism may involve a structural change in attached myosin heads and/or their attachments on heat absorption.