ANK2 Hypermethylation in Canine Mammary Tumors and Human Breast Cancer

• Published in: International journal of molecular sciences Vol. 21; no. 22; p. 8697
• Main Authors: Schabort, Johannes J, Nam, A-Reum, Lee, Kang-Hoon, Kim, Seok Won, Lee, Jeong Eon, Cho, Je-Yoel
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.11.2020; MDPI
• Subjects: Animals; Ankyrins; Basic Helix-Loop-Helix Transcription Factors - metabolism; biomarker; Biomarkers; Biopsy; Breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; cfDNA; CMT; CpG islands; Deoxyribonucleic acid; Disease; DNA; DNA methylation; Dogs; Epigenetics; Female; Gene expression; Genomes; HBC; Humans; hypermethylation; Mammary Neoplasms, Animal - metabolism; Mammary Neoplasms, Animal - pathology; Methylation; Neoplasm Proteins - metabolism; Oncology; Plasma; Trends; Tumors; HBC; CMT; biomarker; cfDNA; hypermethylation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21228697

Canine mammary tumors (CMT) constitute the most common tumor types found in female dogs. Understanding this cancer through extensive research is important not only for clinical veterinary applications, but also in the scope of comparative oncology. The use of DNA methylation as a biomarker has been noted for numerous cancers in the form of both tissue and liquid biopsies, yet the study of methylation in CMT has been limited. By analyzing our canine methyl-binding domain sequencing (MBD-seq) data, we identified intron regions of canine and as differentially methylated regions (DMGs) in CMT. Subsequently, we established quantitative methylation specific PCR (qMSP) of and to validate the target hypermethylation in CMT tissue, as well as cell free DNA (cfDNA) from CMT plasma. Both and were hypermethylated in CMT and highlighted as potential tissue biomarkers in CMT. additionally showed significant hypermethylation in the plasma cfDNA of CMT, indicating that it could be a potential liquid biopsy biomarker as well. A similar trend towards hypermethylation was indicated in HBC at a specific CpG of the target on the orthologous human region, which validates the comparative approach using aberrant methylation in CMT.