Enzyme activities in endothelial cells and smooth muscle cells from swine aorta

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 76; no. 6; pp. 2532 - 2535
• Main Authors: Hayes, Lyle W., Goguen, Cheryl A., Stevens, Arthur L., Magargal, Wells W., Slakey, Linda L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.06.1979; National Acad Sciences
• Subjects: Acetylglucosaminidase - metabolism; Alkaline Phosphatase - metabolism; alpha-Glucosidases - metabolism; Animals; Aorta; Aorta - enzymology; Cathepsins - metabolism; Cell membranes; Cultured cells; Cytochromes; Electron Transport Complex IV - metabolism; Endoplasmic Reticulum - enzymology; Endothelial cells; Endothelium - enzymology; Enzyme activity; Enzymes; Monoamine Oxidase - metabolism; Muscle, Smooth - enzymology; NADH, NADPH Oxidoreductases - metabolism; NADPH-Ferrihemoprotein Reductase - metabolism; Nucleotides - metabolism; Oxidases; Smooth muscle myocytes; Subcellular Fractions - enzymology; Tissue culture techniques
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.76.6.2532

In populations of cultured arterial endothelial and smooth muscle cells grown under the same conditions, we have measured the total activity per cell of 10 enzymes commonly used as ``markers'' for subcellular organelles: NADH: ferricyanide reductase, NADH:cytochrome c reductase (rotenone insensitive), NADPH:cytochrome c reductase, α -glucosidase, 5′-nucleotidase, alkaline phosphodiesterase I, cytochrome oxidase, monoamine oxidase, cathepsin D, and N-acetyl-β -glucosaminidase. Significant differences between the cell types were found for 7 of the 10 enzymes tested. The total activity of 5′-nucleotidase in cultured smooth muscle cells was 17 times that of cultured endothelial cells. Comparison of the activities in the two cell types freshly collected and in culture showed that the difference in 5′-nucleotidase in cultured cells is due principally to loss of activity from endothelial cells, suggesting that this activity is regulated differently in the two cell types. In both cell types cathepsin D activity rose during culture.