Transcriptome Analysis of Flounder (Paralichthys olivaceus) Gill in Response to Lymphocystis Disease Virus (LCDV) Infection: Novel Insights into Fish Defense Mechanisms

• Published in: International journal of molecular sciences Vol. 19; no. 1; p. 160
• Main Authors: Wu, Ronghua, Sheng, Xiuzhen, Tang, Xiaoqian, Xing, Jing, Zhan, Wenbin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.01.2018; MDPI
• Subjects: Actin; Actins - genetics; Actins - metabolism; Animals; Antiviral agents; Apoptosis; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Cell proliferation; Cytokines - genetics; Cytokines - metabolism; differentially expressed genes; DNA Virus Infections - genetics; DNA Virus Infections - metabolism; DNA Virus Infections - virology; Fish; Fish Diseases - genetics; Fish Diseases - metabolism; Fish Diseases - virology; Fish Proteins - genetics; Fish Proteins - metabolism; Flounder - genetics; Flounder - metabolism; Flounder - virology; Function analysis; Gene expression; Genes; Gills - metabolism; Host-pathogen interactions; Immune response; Immune system; Infections; Iridoviridae - pathogenicity; Lymphocystis; lymphocystis disease virus; Metabolism; Molecular modelling; Paralichthys olivaceus; Proteasomes; Proteins; Toll-like receptors; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; Transcriptome; transcriptome sequencing; Ubiquitin; differentially expressed genes; lymphocystis disease virus; transcriptome sequencing; Paralichthys olivaceus
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19010160

Lymphocystis disease virus (LCDV) infection may induce a variety of host gene expression changes associated with disease development; however, our understanding of the molecular mechanisms underlying host-virus interactions is limited. In this study, RNA sequencing (RNA-seq) was employed to investigate differentially expressed genes (DEGs) in the gill of the flounder ( ) at one week post LCDV infection. Transcriptome sequencing of the gill with and without LCDV infection was performed using the Illumina HiSeq 2500 platform. In total, RNA-seq analysis generated 193,225,170 clean reads aligned with 106,293 unigenes. Among them, 1812 genes were up-regulated and 1626 genes were down-regulated after LCDV infection. The DEGs related to cellular process and metabolism occupied the dominant position involved in the LCDV infection. A further function analysis demonstrated that the genes related to inflammation, the ubiquitin-proteasome pathway, cell proliferation, apoptosis, tumor formation, and anti-viral defense showed a differential expression. Several DEGs including , toll-like receptors, cytokine-related genes, antiviral related genes, and apoptosis related genes were involved in LCDV entry and immune response. In addition, RNA-seq data was validated by quantitative real-time PCR. For the first time, the comprehensive gene expression study provided valuable insights into the host-pathogen interaction between flounder and LCDV.