The Effects of In Vivo Exposure to Copper Oxide Nanoparticles on the Gut Microbiome, Host Immunity, and Susceptibility to a Bacterial Infection in Earthworms

• Published in: Nanomaterials (Basel, Switzerland) Vol. 10; no. 7; p. 1337
• Main Authors: Swart, Elmer, Dvorak, Jiri, Hernádi, Szabolcs, Goodall, Tim, Kille, Peter, Spurgeon, David, Svendsen, Claus, Prochazkova, Petra
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.07.2020; MDPI
• Subjects: Antimicrobial agents; Bacteria; Bacterial diseases; Bacterial infections; Biocides; Copper oxides; Exposure; Gene expression; Gram-positive bacteria; Immune response; Immune system; Immunity; infection; Infections; Innate immunity; Intestinal microflora; Loam soils; microbiome; Microbiomes; mRNA; Nanomaterials; Nanoparticles; Nanotechnology; Pathogens; Pesticides; Safety; Soil bacteria; Soil microorganisms; Soils; survival; Worms; United Kingdom > UK; infection; nanoparticles; innate immunity; survival; copper; nanomaterials; Eisenia fetida; microbiome; earthworms
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano10071337

Nanomaterials (NMs) can interact with the innate immunity of organisms. It remains, however, unclear whether these interactions can compromise the immune functioning of the host when faced with a disease threat. Co-exposure with pathogens is thus a powerful approach to assess the immuno-safety of NMs. In this paper, we studied the impacts of in vivo exposure to a biocidal NM on the gut microbiome, host immune responses, and susceptibility of the host to a bacterial challenge in an earthworm. were exposed to CuO-nanoparticles in soil for 28 days, after which the earthworms were challenged with the soil bacterium . Immune responses were monitored by measuring mRNA levels of known earthworm immune genes. Effects of treatments on the gut microbiome were also assessed to link microbiome changes to immune responses. Treatments caused a shift in the earthworm gut microbiome. Despite these effects, no impacts of treatment on the expression of earthworm immune markers were recorded. The methodological approach applied in this paper provides a useful framework for improved assessment of immuno-safety of NMs. In addition, we highlight the need to investigate time as a factor in earthworm immune responses to NM exposure.