An Effective Method of RNA Extraction from Bacteria Refractory to Disruption, Including Mycobacteria

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 µg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was dete...

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Bibliographic Details
Published inNucleic acids research Vol. 25; no. 3; pp. 675 - 676
Main Authors Mangan, J. A., Sole, K. M., Mitchison, D. A., Butcher, P. D.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.02.1997
Subjects
Base Sequence
Detergents - pharmacology
Escherichia coli Proteins
HSP70 Heat-Shock Proteins - genetics
Molecular Sequence Data
Mycobacterium bovis
Mycobacterium bovis - drug effects
Mycobacterium bovis - genetics
RNA, Bacterial - isolation & purification
RNA, Messenger - analysis
Streptococcus pneumoniae - genetics
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Summary:A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 µg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.3.675