The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells
Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of in bovine GCs, and assess whether silencing affected GCs response to melatonin. We found that silencing significan...
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Published in | International journal of molecular sciences Vol. 19; no. 7; p. 2028 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
12.07.2018
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of
in bovine GCs, and assess whether
silencing affected GCs response to melatonin. We found that
silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of
silencing on steroids synthesis, it was found that the expression of
and
enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (
,
,
and
) were up-regulated without affecting the expression of
, suggesting that
silencing may regulate hormone abundance. Furthermore,
silencing significantly increased the expression of
and
, and decreased the expression of
and
without significant difference in the expression of
and
. In addition,
silencing didn't affect the effect of melatonin on increasing the expression of
,
,
and
, and progesterone level, or decreasing
,
and
expression, and production of inhibin B. Moreover,
silencing could disrupt the role of melatonin in decreasing the
,
and
expression, and activin B secretion. In conclusion, these results reveal that melatonin and MT2 are essential regulator of bovine GCs function by modulating reproduction-related genes expression, hormones secretion and other regulators of folliculogenesis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms19072028 |