Evaluation of the Biological Behavior of a Gold Nanocore-Encapsulated Human Serum Albumin Nanoparticle (Au@HSANP) in a CT-26 Tumor/Ascites Mouse Model after Intravenous/Intraperitoneal Administration

• Published in: International journal of molecular sciences Vol. 20; no. 1; p. 217
• Main Authors: Chen, Chao-Cheng, Li, Jia-Je, Guo, Nai-Hua, Chang, Deng-Yuan, Wang, Chung-Yih, Chen, Jenn-Tzong, Lin, Wuu-Jyh, Chi, Kwan-Hwa, Lee, Yi-Jang, Liu, Ren-Shyan, Chen, Chuan-Lin, Wang, Hsin-Ell
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.01.2019; MDPI
• Subjects: Abdomen; Adenocarcinoma; Administration, Intravenous; Albumin; Animals; Annual reports; Area Under Curve; Ascites; Ascites - pathology; Body weight; Breast cancer; Cancer therapies; Cell Survival; Chemotherapy; Chromatography; Colon; Colon cancer; Colorectal cancer; Colorectal carcinoma; Computed tomography; Cytotoxicity; Disease Models, Animal; Drug delivery systems; Drug dosages; Dynamic Light Scattering; Gold - administration & dosage; Human behavior; Human serum albumin; Hybrid protein-inorganic nanoparticle; Indium Radioisotopes - chemistry; Indium Radioisotopes - pharmacokinetics; Injections, Intraperitoneal; Injections, Intravenous; intraperitoneal injection; Intravenous administration; intravenous injection; Liver; Lung cancer; Medical diagnosis; Medical prognosis; Metastases; Metastasis; Mice; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - ultrastructure; NMR; Nuclear magnetic resonance; Paclitaxel; Pancreas; Particle Size; Pharmacokinetics; Pharmacology; Proteins; Serum albumin; Serum Albumin, Human - administration & dosage; Serum Albumin, Human - pharmacokinetics; Spleen; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; tumor/ascites animal model; Hybrid protein-inorganic nanoparticle; intraperitoneal injection; tumor/ascites animal model; intravenous injection
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20010217

Colorectal cancer is one of the major causes of cancer-related death in Taiwan and worldwide. Patients with peritoneal metastasis from colorectal cancer have reduced overall survival and poor prognosis. Hybrid protein-inorganic nanoparticle systems have displayed multifunctional applications in solid cancer theranostics. In this study, a gold nanocore-encapsulated human serum albumin nanoparticle (Au@HSANP), which is a hybrid protein-inorganic nanoparticle, and its radioactive surrogate In-labeled Au@HSANP ( In-Au@HSANP), were developed and their biological behaviors were investigated in a tumor/ascites mouse model. In-Au@HSANP was injected either intravenously (iv) or intraperitoneally (ip) in CT-26 tumor/ascites-bearing mice. After ip injection, a remarkable and sustained radioactivity retention in the abdomen was noticed, based on microSPECT images. After iv injection, however, most of the radioactivity was accumulated in the mononuclear phagocyte system. The results of biodistribution indicated that ip administration was significantly more effective in increasing intraperitoneal concentration and tumor accumulation than iv administration. The ratios of area under the curve (AUC) of the ascites and tumors in the ip-injected group to those in the iv-injected group was 93 and 20, respectively. This study demonstrated that the ip injection route would be a better approach than iv injections for applying gold-albumin nanoparticle in peritoneal metastasis treatment.